?????????????????????????
????嶾??Microcystin ELISA?????
?????????м??
?????????????????????????嶾??-LR (MC-LR)??BSA?????????????????????????????MC-LR?????????(MC8C10)????????????MC-LR??????ELISA???????С?
??????п????????????嶾??-LR???????????????????(???????????????????)????????????????????н??ж??????
????????????????????λ????????????嶾???????н??淴????????λ????????????嶾???н?С????淴?(≤10%)??
??????????????0.1μg/L?????????????0.2 ~4.0μg/L??
???????????????≤10%??
??????~2h
???????????20~40oC
????????????
1) ???????? 1??
2) 96??????? 1?? (8??/?? × 12??) (??????MC-LR-BSA)
3) MC-LR???? 5? (2mL/?) (0.2μg/L, 0.5μg/L, 1.0μg/L, 2.0μg/L, 4.0μg/L)
4) ????嶾??-LR???? 1? (7mL)
5) ????????? 1? (12mL)
6) ????????A 1? (6mL)
7) ????????B 1? (6mL)
8) ???? 1? (7mL)
9) ???????(20×) 1? (18mL)
???????????????豸
1) ????????????(20~200μL) 1? ???????????????(20~200μL) 1?
2) ?????(??450nm????)??????????????????????????????????
3) 0.45μm??????????????????????
4) 4oC??????
5) ????????????
???????????
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???и?????????????????????,???????? ?????????????????????????????????????????????????????????????????
????????????????
????????QQ??1069267096
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?????010-60206266
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