Key wordsSpirulina platensis; Sulphoquinovosyldiglyceride (SQDG);extraction
AbstractSulphoquinovosyldiglyceride (SQDG) was separated from the total lipid of Spirulina platensis with silic gel and then was further purified into 7 main components by HPLC. Lipase XI from Rhizopus arrhizus was used to hydrolyze the fatty acids esterified in sn-1. The composition of fatty acids in total lipid and SQDG was analyzed by GC after methylation .The results show that the main fatty acids in SQDG are 16∶0，18∶3n-6，18∶2n-6. The contents of 20∶4 and 20∶5 in the SQDG of Spirulina platensis are lower than those of other red algae and brown algae while 16∶2 was undiscovered. The mole-cular species analysis of SQDG shows that the unsaturated fatty acids such as C18∶2n-6 and C18∶3n-6 mainly occupy the sn-1 position, while saturated fatty acid C16:0 in the sn-2 position. The composition of SQDG is mainly 52%~57% (18∶2,16∶0), 11% (16∶0,16∶0), 7.1%(16∶1,16∶0), 7.7%(18∶1,16∶0).
(2 Product Inspection and Supervision Department of Qingdao, Qingdao 266003, China)
(1 College of Fisheries, Ocean University of Qingdao, Qingdao 266003, China)
Cao Yang1 Hu Yaqin1 Zhao Xue1 Li Zhaoyong2
The Molecular Species Composition of Sulphoguinovosyldiglycerides in Spirulina platensis
中图法分类号Q546；Q949.2 文章编号1001-1862(2002)05-0727-07
关键词螺旋藻；硫酸喹诺糖二酰甘油酯(SQDG)；提取(分离)
摘 要 本文采用硅胶柱层析法，从螺旋藻总脂中分离提取出硫酸喹诺糖二酰甘油酯(SQDG)，通过高压液相色谱(HPLC)分离纯化得到7个主要组分。采用气相色谱法，分析SQDG中脂肪酸的含量和分子组成。结果表明，SQDG中主要脂肪酸为16∶0，18∶3n-6，18∶2n-6，而花生四稀酸(20∶4)及EPA(20∶5)的含量要低于其他几种红藻和褐藻，未发现16∶2。SQDG中sn-1位79%为不饱和脂肪酸，95%以上的sn-2位脂肪酸为16∶0。根据测得的7种组分的含量和sn-1和sn-2位脂肪酸的分析计算可得，SQDG主要分子组成为52%~57%(18∶2,16∶0)，11% (16∶0,16∶0), 7.1% (16∶1,16∶0)，7.7% (18∶1,16∶0)。
(1青岛海洋大学水产学院，青岛，266003)(2青岛市产品质量监督检验所，青岛，266071)
曹杨1 胡亚芹1 赵 雪1 李昭勇2
螺旋藻硫酸喹诺糖二酰基甘油酯脂肪酸分子组成分析