?л????????????????
??
?л?????????
Organic matter-decomposing inoculant
??
??
??
2002-11-05???? 2002-12-20??
?л???????????? ?? ??
??
??
??
? ??
???????????л??????????????GB/T 1.1—2000??????????????? ??1??????????????д?涨?????б?д???
?????????A??淶??????
????????л??????????????????????????
????????л?????????????????
?????????λ????????????????????????????????????????????????????
??????????????????????? ?????? ?????????????????? ???
?л?????????
1????Χ
??????涨???л????????????????????????????????????塢??????????????鷽?????????????????????????????????
???????????????????л??????????????????????????????︴??????????????????
2???淶?????????
????????е????????????????????????????????????????????????????????????е?????????????????????????????????????????????????????????????????Э???????о??????????Щ????????°汾???????????????????????????°汾????????????
GB 4789.3 ???????????? ???????????
GB 7959 ???????????????
GB/T 14539.1 ??????????顢???????
GB/T 14539.2 ?????????????????
GB/T 14539.3 ?????????????????
GB/T 14539.4 ?????????????????
GB/T 15555.1 ??????? ?????? ??????????·?????
GB/T 15555.5 ??????? ?????? ??????????????
NY 227 ????????
NY 411 ?????????
QB/T 1803 ?????????????鷽??
3??????????
?л????????????????????л?????(?????????????????????????????????????)?????????????????????
4?????????
???????????????????塢?????????????????
5?????
5.1??????
????????????????????????Ч?????????????????λ????????????估????????????
5.2??????????5.3????5.4??
??????5.1???
??5.1 ??????????
|
?? ? |
?? ?? |
|||
|
? ?? |
?? ?? |
?? ?? |
||
|
?? ?? |
|
?????ζ??? |
??????????? |
?????????????е???? |
|
??Ч?????(cfu),??/g(mL) |
≥ |
1.0 |
0.50 |
0.50 |
|
? ???% |
≤ |
- |
35.0 |
20.0 |
|
???????? U/mL |
≥ |
30.0 |
30.0 |
30.0 |
|
???????U/g??mL?? |
≥ |
15.0 |
15.0 |
15.0 |
|
??????U/g??mL?? |
≥ |
10.0 |
10.0 |
10.0 |
|
pH |
|
5.0??8.5 |
5.5??8.5 |
5.5??8.5 |
|
??Ч????? |
≥ |
6 |
12 |
|
|
???????????????????????????????? |
||||
??
5.5?????????????
??????5.2???
??5.2 ???????????
|
?? ?? |
?? |
??????? |
|
?????? ??/g(mL) |
≤ |
1000 |
|
??????????? % |
≥ |
95 |
|
????????????Hg??? mg/kg?? |
≤ |
5 |
|
???????????Cd???mg/kg?? |
≤ |
10 |
|
????????????Cr???mg/kg?? |
≤ |
150 |
|
?鼰????????As???mg/kg?? |
≤ |
75 |
|
???????????Pb???mg/kg?? |
≤ |
100 |
|
????????????????????????????????????????????????????????? |
||
6??????
??????????????????????????????????????????????ν??г??????顣??????????????????????
6.1??????????
???????????????????????????????????????????????????????????????
6.2????????????????
???????г???????????????????
??????????λ??С?????2kg??1000mL???￡??????????????????????????3?????????????????1?????????????3?????????????????С??500g(mL)????????????￡????鼸?????????????????????ɡ???????30~50kg??????????????????????????????????5??????????????￡???????????500g??mL????????????????????????????3???????????????????????500g(mL)??
7???????
7.1?????
??????5.1?е????
7.2????Ч???????
?????NY 227?е?涨??
7.3?????????
?????NY 227?е?涨??
7.4??pH???
?????NY 227?е?涨??
7.5???????????????
7.5.1??????????
??????GB4789.3??涨??
7.5.2????????????????
?????GB 7959 ??涨??
7.5.3???麬????
?????GB/T 14539.2??涨??
7.5.4?????????
?????GB/T 14539.3??涨??
7.5.5?????????
?????B/T 14539.4??涨??
7.5.6??????????
?????GB/T 15555.1??涨??
7.5.7??????????
?????GB/T 15555.5??涨??
7.6????????
??????A??涨??
8?????????
8.1?????????
8.1.1?????????飨??????飩
?????????????????????????????鲿???????5.1???м??飬???????????????????????????????????????????????Ч???
8.1.2????????飨???м??飩
???????￡?????????????Ρ????????????????????????????顣
a. ?2????????
b. ???????????????н???????仯??
c. ???????????????????????н????????
d. ?????????????????г???????顣
8.2???ж?????
8.2.1?????????κ?????????????????
a)??????????????????????????????
b)?????Ч???????????????????????????pH?????????????????????????????????????????У???2?????????2???????????
8.2.2?????????κ??????????????????
a)???????Ч?????????????????
b)?????????pH?????????????????????????????????????????У???3?????????????????????
c)??????????????????????????
??
9?????????10???????????????
?л????????????????????????????水NY411??9.1??9.2??9.3??9.4??С?
???????A
???淶??????
?????
???????A
???淶??????
?????
A. ????????
A.1 ????????
a) ??????????????0.625g????????????Σ?????100.0mL????????????0.2 M, pH 6.0??,????????????
b) DNS??????????10.0g 3,5-?????????????????????У?????20.0g ?????????200.0g ?????????500.0mL?????????????????????2.0g?????????????0.5g????????????????????1000.0mL??
A.2 ?????豸
a) ??????
b) ???????
c) ????
d) ??????
A.3 ??????????
a) ????????????????????????25.0mg???????????25.0mL??
b) ???????????
?5?????20.0mL???????????±?????????
????и???1.0mL 2.0 M?????????????2.0mL DNS?????????????????????????5.0 min?????????????????????20.0mL????????490nm?????????OD????????????μg?????????OD???????????????????
A.4 ?????
???5.0g?????????????10??????????????30min??????????????????????
A.5 ??????
?3?????20.0mL????????1???????????????2???????????????????м?1.0mL??????????3?????з?????4.0mL???????60?????????????60????????з??20min????????????????1.0mL 2.0M?????????????2.0mLDNS???????????????????????1.0mL?????????3???????????У????5min?????????????????????????20.0mL???÷???????490nm??????OD???
A.6 ??????????????
????????????????????μg????
??
U = ??M1-M0??/ 20
??У?
U??????????????μg/min??
M0????????????????μg??
M1???????????????μg??
20????????????min??
1mL???????????????1μg??????????1?????????λ??u????
B. ?????????
B.1 ???
?????QB/T 1803?е?涨??
B.2 ??????????
??????10.0g????10.0mL??????????в?????????????150mL???У???????????????????????pH7.5?????200?/min?????30min???????????????????????3000?/min????20min??????????????????????????????pH7.5?????????????????????á?
B.3 ?????QB/T 1803??涨
???????????????????????????????????????10min???30min????????????????????5min?????
C. α-???????
C.1 ???
?????QB/T 1803?е?涨??
C.2 ??????????
??????10.0g????10.0mL??????????в?????????????150mL???У????????????????????????200?/min?????30min???????????????????????3000?/min????20min???????????????????????????????????????????????á?
C.3 ???????????
a????????????????????±????á?
b???????????????1.0mL????????????飩??????5mL?????У???????660nm???????,??????????????0???????????????????????????????????????????????????????????????????
????????????鷽??????????????1??????????????mg?????????????K?????????0.95~1.00??Χ???
C.4 ????QB/T 1803-1993????????в???
???????????????????????б?????????2%???????????20.0mL???1%???????????10.0mL????????????λ??????10.0mL???5.0mL?????????????5min???30min??
C.5 ????
X= ( 4.76-A×K ) ×21×n/30
???У?
X??????????????u/g(mL)??
4.76??1mL????????????е?????????????mg????
A??????????????????????
K??????????
21???????????????mL??
n ??????????
30????????30min??
1g??mL?????????60?桢pH=6.0?????￡?1???????1mg????????????1?????????λ????u/g(mL)?????
