涓€銆佹煡鏂板挩璇㈣鐐癸細锛堜富瑕佺爺绌跺唴瀹广€佹妧鏈柟娉曘€佹妧鏈寚鏍囥€佸垱鏂扮偣锛?/span>

濮旀墭浜鸿棰樻帰绱㈠缓绔嬪揩閫熷彲闈犵殑妫€娴嬫敮鍘熶綋鎰熸煋鐤剧梾鐨勬娴嬫柟娉曘€?span style="text-shadow: auto">杩愮敤澶氶噸瀹炴椂鑽у厜瀹氶噺PCR鎶€鏈?/span>锛岄噰鐢ㄩ€氱敤寮曠墿鍜岀壒寮傚紩鐗┿€佹帰閽堬紝寤虹珛鍚屾椂妫€娴嬫敮鍘熶綋鍜屽疄楠屽姩鐗╁繀椤绘帓闄ょ殑涓夌甯歌鏀師浣撹偤鏀師浣撱€佸叧鑺傜値鏀師浣撱€佹憾绁炵粡鏀師浣撶殑鏂规硶銆傞€氳繃鍚岀涓€娆℃娴嬪氨鑳藉緱鍒扮粨鏋滐紝涓烘棭鏈熴€佸揩閫熻瘖鏂彁渚涙湁鏁堟墜娈碉紝浠ヤ究鏃╂湡鍙婃椂鍙戠幇鎰熸煋鎯呭喌銆備笉浠呰兘瀵圭粷澶у鏁版敮鍘熶綋鐨勬€诲惈閲忚繘琛屽噯纭殑瀹氶噺锛岃€屼笖杩樿兘瀵逛笁绉嶅父瑙佺殑瀹為獙鍔ㄧ墿蹇呴』鎺掗櫎鐨勬敮鍘熶綋杩涜鍑嗙‘鐨勫畾閲忋€傝鏂规硶鐨勫缓绔嬪強鍏跺湪瀹為獙鍔ㄧ墿鏀師浣撴劅鏌撹瘖鏂笂鐨勫簲鐢紝蹇呭皢瀵瑰疄楠屽姩鐗╂劅鏌撶柧鐥呯殑璇婃柇浜х敓閲嶈褰卞搷銆傚悓鏃朵篃涓轰汉绫讳复搴婂揩閫熻瘖鏂敮鍘熶綋鎰熸煋鎻愪緵浜嗕竴绉嶆柊棰栫殑鏂规硶锛屼负鎺ㄥ姩璇ユ娴嬮鍩熸按骞崇殑鎻愰珮浣滄垚鍑鸿础鐚€?/span>

浜屻€佹煡鏂板挩璇㈣姹傦細

1銆佹煡鏄庝笌璇ョ爺绌堕」鐩浉鍚屾垨绫讳技鐨勫浗鍐呭鐮旂┒鎶ラ亾锛?o:p>

2銆佸皢璇ョ爺绌跺唴瀹逛笌鍥藉唴澶栫爺绌惰繘琛屽姣斿垎鏋愶紱

3銆佸璇ョ爺绌堕」鐩殑鏂伴鎬т綔鍑哄垎鏋愯瘎浠枫€?o:p>

涓夈€佹绱㈡儏鍐碉細

1銆佹绱㈣寖鍥达細鍥藉唴澶?o:p>

2銆佹绱㈠伐鍏凤細

锛?锛夊浗澶栨枃鐚绱㈠伐鍏凤細

鈻?span lang="EN-US">INTERNET-PUBMED锛?span lang="EN-US">MEDLINE

鈻?/span>缇庡浗鍖诲鏂囩尞鍏夌洏妫€绱㈢郴缁?/span>(MEDLINE-CDROM)

锛?span lang="EN-US">2锛?/span>鍥藉唴鏂囩尞妫€绱㈠伐鍏?/span>锛?span lang="EN-US">

鈻?/span>涓浗鐢熺墿鍖诲鏂囩尞鍏夌洏妫€绱㈢郴缁?/span>(CBM-disc)

鈻燙HKD璺ㄥ簱妫€绱㈠钩鍙帮紙鏈熷垔鍏ㄦ枃搴撱€佸崥纭曡鏂囧叏鏂囧簱銆佷細璁叏鏂囧簱锛?o:p>

鈻?/span>涓枃绉戞妧鏈熷垔鏁版嵁搴擄紙缁存櫘锛?/span>

鈻?/span>涓囨柟鏁板瓧鍖栨湡鍒婂叏鏂囨暟鎹簱

鈻?/span>鍥藉绉戞妧鎴愭灉搴?o:p>

鈻?/span>鍥藉绉戞妧鍥句功鏂囩尞涓績锛?/span>NSTL锛?/span>

锛?锛夌敓鐗╁尰瀛﹀弬鑰冧功銆佸伐鍏蜂功锛?o:p>

锛?锛夌幇鍒婏細

3銆佹绱㈡墜娈碉細 缃戠粶妫€绱?span style="mso-spacerun: yes">    鍏夌洏妫€绱?span style="mso-spacerun: yes">    鎵嬪伐妫€绱?o:p>

4銆佹绱㈤€斿緞锛?涓婚閫斿緞 銆€ 鑷敱璇嶉€斿緞

5銆佹绱㈢敤璇嶏細

鈶?/span>"鏀師浣撳睘/鍏ㄩ儴鍓富棰?span lang="EN-US">/鍏ㄩ儴鏍?span lang="EN-US">" OR"鏀師浣撶/鍏ㄩ儴鍓富棰?span lang="EN-US">/鍏ㄩ儴鏍?span lang="EN-US">" OR "鏀師浣撶洰/鍏ㄩ儴鍓富棰?span lang="EN-US">/鍏ㄩ儴鏍?span lang="EN-US">" OR  鏀師浣?/span>(mycoplasma OR Mycoplasmataceae OR Mycoplasmatales)

鈶?/span>"鑱氬悎閰堕摼鍙嶅簲/鍏ㄩ儴鍓富棰?span lang="EN-US">/鍏ㄩ儴鏍?span lang="EN-US">" OR PCR(polymerase chain reaction)

鈶?/span>鑽у厜瀹氶噺 OR 瀹氶噺鑽у厜(fluorescent quantitation锛?span lang="EN-US">

鈶?/span>"鏀師浣?span lang="EN-US">, 鑲?/span>/鍏ㄩ儴鍓富棰?span lang="EN-US">/鍏ㄩ儴鏍?span lang="EN-US">" OR "鏀師浣?span lang="EN-US">, 鑲虹値/鍏ㄩ儴鍓富棰?span lang="EN-US">" OR 鑲烘敮鍘熶綋锛?span lang="EN-US">Mycoplasma pulmonis OR Mycoplasma pneumoniae锛?span lang="EN-US">

鈶?/span>"鏀師浣?span lang="EN-US">, 鍏宠妭鐐?/span>/鍏ㄩ儴鍓富棰?span lang="EN-US">/鍏ㄩ儴鏍?span lang="EN-US">" OR 鍏宠妭鐐庢敮鍘熶綋锛?span lang="EN-US">Mycoplasma arthritidis锛?span lang="EN-US">

鈶?/span>婧剁缁忔敮鍘熶綋(Mycoplasma neurolyticum)

6銆佹绱㈢瓥鐣?/span>锛?span lang="EN-US">

#1 鈶?AND  鈶?span lang="EN-US"> AND 鈶?/span>

#2 鈶?span lang="EN-US"> AND 鈶?/span> AND 鈶?/span> AND 鈶?/span> AND 鈶?/span>

鍥涖€佹绱㈢粨鏋?/span>锛?/span>

1銆佹绱㈢粨鏋滄€昏〃

2銆佸悇妫€绱㈠伐鍏锋绱㈡儏鍐典竴瑙堣〃锛?o:p>

3銆佺浉鍏虫枃鐚?o:p>

1.         鏉ㄦ枃鍚?/span>; 寮犲缓鑺?/span>; 鏉庣嚂;绛?/span>(鍖椾含澶у涓村簥鑲跨槫瀛﹂櫌鍖椾含甯傝偪鐦ら槻娌荤爺绌舵墍,100034) . 鍏鏀師浣撳崟鍏嬮殕鎶椾綋鐨勫埗澶囦笌閴村畾. 涓崕妫€楠屽尰瀛︽潅蹇?/span>  2006; 29(2): 156-159

鏉ㄦ枃鍚?/span>绛?/span>鍒跺浜嗛拡瀵逛复搴婂父瑙佺殑鍏鏀師浣撶殑鐗瑰紓鎬у崟鍏嬮殕鎶椾綋,涓轰复搴婃敮鍘熶綋鎰熸煋妫€娴嬫彁渚涘伐鍏枫€傛柟娉?/span>:鐢ㄧ伃娲荤殑鏀師浣撻绮掑厤鐤?/span>BALB/c灏忛紶,閫氳繃浼犵粺鏉備氦鐦ゆ妧鏈埗澶囨姉鏀師浣撳崟鍏嬮殕鎶椾綋,閲囩敤6绉嶆敮鍘熶綋铔嬬櫧浠ラ叾鑱斿厤鐤惛闄勮瘯楠?/span>(ELISA)杩涜浜ゅ弶绛涢€?/span>,骞剁敤鍏嶇柅鍗拌娉?/span>(WB)瀵瑰叾鐗瑰紓鎬ц繘琛岄壌瀹氥€傜粨鏋?/span>:鑾峰緱閽堝鐢熸畺鏀師浣撱€佹ⅷ褰㈡敮鍘熶綋銆佺尓榧绘敮鍘熶綋銆佸彂閰垫敮鍘熶綋銆佽偤鐐庢敮鍘熶綋銆佺┛閫忔敮鍘熶綋鐨勭壒寮傛€у崟鍏嬮殕鎶椾綋鍚?/span>1鏍?/span>,缁?/span>ELISA妫€娴嬭瘉瀹炲叿鏈夎緝濂界殑鍙嶅簲鐗瑰紓鎬?/span>,鍏朵腑,鍗曟姉Mg6H1銆?/span>Mpri ZW1鍜?/span>Mpe6G4鍦?/span>WB涓篃鍚屾椂鍒嗗埆涓庣敓娈栨敮鍘熶綋銆佹ⅷ褰㈡敮鍘熶綋鍜岀┛閫忔敮鍘熶綋鍛堢壒寮傚弽搴斻€傜粨璁?/span>:鑾峰緱閽堝6绉嶄汉浣撳父瑙佹劅鏌撴敮鍘熶綋鐨勪笉鍚屽崟鍏嬮殕鎶椾綋,瀹冧滑鍦?/span>ELISA妫€娴?/span>涓叿杈冨ソ鐨勫弽搴旂壒寮傛€?span lang="EN-US">,鍙簲鐢ㄤ簬鏀師浣撴劅鏌撴娴嬨€?/span>

2.       鐜嬭緣; 鐜嬬幃钃?span lang="EN-US">; 娈甸€哥兢;绛?/span>锛?/span>姝︽眽甯傜涓€鍖婚櫌鐨偆绉?span lang="EN-US"> 430022锛? 鍙嶅悜绾跨偣鏉備氦鏂规硶妫€娴嬪拰閴村畾4绉嶅父瑙佽嚧鐥呮敮鍘熶綋. 涓浗鐨偆鎬х梾瀛︽潅蹇?span lang="EN-US">  2004.11.20; 18(11): 692-693

鐜嬭緣绛?/span>璇勪环浜嗗弽鍚戠嚎鐐规潅浜ゆ柟娉曟娴嬬敓娈栨敮鍘熶綋銆佷汉鍨嬫敮鍘熶綋銆佸井灏忚劜鍘熶綋鍜岃В鑴茶劜鍘熶綋鐨勬晱鎰熸€с€傛柟娉?/span>:搴旂敤鏀師浣撳弽鍚戠嚎鐐规潅浜ゆ柟娉曞拰绉嶇壒寮傛€?/span>PCR鏂规硶鍚屾椂妫€娴?/span>198渚嬩复搴婃爣鏈?/span>,姣旇緝涓ょ鏂规硶鐨勬娴嬬粨鏋溿€傜粨鏋?/span>:鏀師浣撳弽鍚戠嚎鐐规潅浜ゆ柟娉曞拰绉嶇壒寮傛€?/span>PCR妫€娴嬫敮鍘熶綋闃虫€х巼鍒嗗埆涓?/span>33.3%鍜?/span>34.3%,涓ょ鏂规硶妫€娴嬬粨鏋滅鍚堢巼涓?/span>98.5%銆?/span>3渚嬫爣鏈敮鍘熶綋绉嶇壒寮傛€?/span>PCR鏂规硶妫€娴嬪井灏忚劜鍘熶綋闃虫€?/span>,鑰屽弽鍚戠嚎鐐规潅浜ゆ柟娉曟娴嬩负闃存€с€備袱绉嶆柟娉曟娴嬫敮鍘熶綋缁撴灉鏃犳樉钁楁€у樊寮?/span>(P>0.05)銆傜粨璁?/span>:鍙嶅悜绾?/span>鐐规潅浜ゆ柟娉曡兘鍚屾椂蹇€熴€佹晱鎰熷拰鐗瑰紓鐨勬娴嬭繖4绉嶆敮鍘熶綋銆?/span>

3.         绯滅鐓?span lang="EN-US">; 绉︾幉锛?/span>鏃犻敗甯傚厠闅嗛仐浼犳妧鏈爺绌舵墍 214026锛? 鍙戦叺鏀師浣撱€佺┛閫氭敮鍘熶綋鍙岄噸濂楀紡PCR妫€娴嬬爺绌?/span>. 涓浗浜哄吔鍏辨偅鐥呮潅蹇?span lang="EN-US">  2000.07.15; 16(4): 50-52

绯滅鐓?/span>绛?/span>搴旂敤鍙岄噸濂楀紡PCR鎶€鏈悓鏃舵娴嬪彂閰垫敮鍘熶綋(Mf)鍜岀┛閫氭敮鍘熶綋(Mpe)銆備互鏀師浣?/span>16SvRNA鍩哄洜涓洪澏鍩哄洜,澶栧寮曠墿涓?/span>Mf涓?/span>Mpe鍏辩敤銆佸唴濂楀紩鐗╁垯鍒嗗埆涓?/span>Mf绉嶇壒寮傚拰Mpe绉嶇壒寮?/span>,寤虹珛鍙岄噸濂楀紡鎵╁浣撶郴銆傜粨鏋?/span>:鏈弻閲嶅寮?/span>PCR涓嶄粎鑳藉垎鍒墿澧?/span>Mf鍜?/span>Mpe鐨勭壒寮傛€?/span>DNA,骞惰兘鍚屾椂鎵╁Mf鍜?/span>Mpe鍑虹幇浜屾潯鐗瑰緛鎬ф潯甯?/span>,浜х墿缁忔祴搴?/span>璇佸疄銆傜粨璁?span lang="EN-US">:鍙岄噸濂楀紡PCR鏄竴绉嶇伒鏁忋€佺壒寮傚拰蹇€熺殑Mf鍜?span lang="EN-US">Mpe妫€娴嬫柟娉曘€?span lang="EN-US">

4.         鍚戝崕鍥?/span>; 鐔婄ぜ瀹?/span>; 鍒樺皬绔?/span>; 绛夛紙娣卞湷甯傜姘稿尰闄?/span>,518103锛?/span>. 鑶滆姱鐗囨妧鏈娴嬫硨灏跨敓娈栭亾娌欑溂琛ｅ師浣撱€佹穻鐥呭鐟熻弻鍜?/span>3绉嶆敮鍘熶綋鐨勬柟娉曞鐮旂┒. 涓崕寰敓鐗╁鍜屽厤鐤鏉傚織  2007; 27(8): 758-761

鍚戝崕鍥界瓑鐮旂┒寤虹珛鍜岃瘎浠蜂竴涓柊鐨勫閲?/span>PCR.鍙嶅悜绾跨偣鏉備氦鎶€鏈?/span>(RIJB)蹇€熷悓鏃舵娴嬫硨灏跨敓娈栭亾娌欑溂琛ｅ師浣?/span>(Ct)銆佹穻鐥呭鐟熻弻(鍧?/span>)鍜?/span>3绉嶆敮鍘熶綋鎰熸煋鐨勬柟娉曘€傛柟娉?/span>:鍒嗗埆閫夋嫨Ct闅愯斀璐ㄧ矑鍜?/span>Ng16SrRNA鍩哄洜璁捐涓ゅ鐗瑰紓鎬у紩鐗?/span>,浠ユ敮鍘熶綋鍐呰浆褰曢棿闅斿簭鍒?/span>(ITS)璁捐涓€瀵规敮鍘熶綋灞為€氱敤寮曠墿,鐢熺墿绱犳爣璁颁笅娓稿紩鐗┿€傛瀯寤轰笁閲?/span>PCR鍚屾椂鎵╁Ct銆?/span>Ng銆佽В鑴茶劜鍘熶綋銆佸井灏忚劜鍘熶綋銆佷汉鍨嬫敮鍘熶綋(Mh)绛夎弻DNA,鐒跺悗涓庡浐瀹氬湪灏奸緳鑶滀笂鐨勫悇鐗瑰紓鎬у鏍歌嫹閰告帰閽堟潅浜ゃ€傚苟瀵?/span>142浠界粡鑽у厜瀹氶噺PCR(FQ-PCR)妫€娴?/span>0鍜屽潧鐨勬€х梾楂樺嵄浜虹兢鏍囨湰,浠ュ強45浠界粡鏀師浣撴恫浣撳煿鍏绘硶閴村畾鐨勬爣鏈繘琛屾娴嬨€傜粨鏋?/span>:澶氶噸PCR鍙悓鏃舵墿澧?/span>Ct銆?/span>Ng銆?/span>Uu銆?/span>Up鍜?/span>Mh鏍囧噯鑿屾牚DNA,鍏?/span>PCR浜х墿鐨勭墖娈甸暱搴︿负208锛?/span>408bp銆?/span>97浠?/span>FQ-PCRCt闃虫€ф爣鏈腑鏈?/span>93浠界粡澶氶噸PCR-RLB妫€娴嬩负Ct闃虫€?/span>,45浠?/span>FQ-PCRCt闃存€ф爣鏈腑35浠藉閲?/span>PCR-RLB闃存€с€?/span>41浠?/span>FQ-PCRNg闃虫€ф爣鏈腑34浠藉閲?/span>PCR-RLBNg闃虫€?/span>,101浠?/span>FQ-PCRNg闃存€ф爣鏈腑98浠藉閲?/span>PCR-RLB闃存€с€傚叾涓?/span>36浠界粡澶氶噸PCR-RLB妫€娴嬩负娣峰悎鎰熸煋銆?/span>32浠芥敮鍘熶綋娑蹭綋鍩瑰吇闃虫€ф爣鏈腑28浠藉閲?/span>PCR-RLB闃虫€?/span>,13浠芥敮鍘熶綋鍩瑰吇闃存€ф爣鏈粡澶氶噸PCR-RLB妫€娴嬪潎涓洪槾鎬с€傜粨璁?/span>:澶氶噸PCR-RLB鍙揩閫熷悓鏃舵娴?/span>Ct銆?/span>Ng銆?/span>Uu銆?/span>Up鍜?/span>Mh,涓烘€т紶鎾柧鐥呯殑涓村簥璇婃柇鎻愪緵浜嗕竴绉嶅彲闈犵殑鏂规硶銆?/span>

5.       瀛欑户姊?/span>; 寮犳櫤娲?/span>; 閭甸槼(娌堥槼涓浗鍖荤澶у绗簩涓村簥瀛﹂櫌妫€楠岀 110003). 瀹炴椂鑽у厜瀹氶噺鑱氬悎閰堕摼鍙嶅簲妫€娴嬫穻鐞冭弻銆佹矙鐪艰。鍘熶綋鍙婅В鑴叉敮鍘熶綋. 涓婃捣鍖诲妫€楠屾潅蹇?/span>  2001.05.30; 16(3): 172-173

   瀛欑户姊?/span>绛?/span>鐢ㄨ崸鍏夊畾閲忚仛鍚堥叾閾惧弽搴?/span>(FQ-PCR)妫€娴嬫硨灏跨敓娈栭亾鎴栧棰堝垎娉岀墿涓穻鐞冭弻(NG)銆佹矙鐪艰。鍘熶綋(CT)銆佽В鑴叉敮鍘熶綋(UU)鐨勬暟閲?/span>,浠ヤ簡瑙ｄ笂杩?/span>3绉嶇梾鍘熶綋鎰熸煋鐜扮姸銆傛柟娉?/span>:浠ヨ崸鍏夋帰閽堟潅浜ゆ妧鏈负鍩虹鐨勫疄鏃跺畾閲?/span>PCR娉曘€傜粨鏋?/span>:鎵€妫€鐨?/span>296浠芥爣鏈腑妫€鍑虹巼鍒嗗埆涓?/span>NG 14.4%(42/296),CT 10.8%(32/296) ,UU 15.5%(46/296),鎬绘鍑虹巼涓?/span>30.1%(89/296)銆傜粨璁?/span>:搴旂敤FQ-PCR鍏锋湁绠€渚裤€佸揩閫熴€佸噯纭殑浼樼偣,1浠芥爣鏈彲鍚屾椂妫€娴?/span>3绉嶇梾鍘熶綋,鏄洰鍓嶅揩閫熻瘖鏂穻鐥?/span>,闈炴穻鑿屾€у翱閬撶値鍙潬鍑嗙‘鐨勬柟娉曘€?/span>

6.       浠囪緟鍚?/span>(鐢樿們鐪佸钩鍑夊競浜烘皯鍖婚櫌, 744000). FQ-PCR妫€娴嬩笉瀛曚笉鑲叉偅鑰呰В鑴叉敮鍘熶綋鍜屾矙鐪艰。鍘熶綋. 涓浗浼樼敓涓庨仐浼犳潅蹇?/span>  2007; 15(5): 46,43

    浠囪緟鍚涢噰鐢ㄥ疄鏃惰崸鍏夊畾閲?/span>PCR 鎶€鏈娴?/span>45瀵逛笉瀛曚笉鑲插か濡囧コ鎬у棰堝垎娉岀墿銆佺敺鎬х簿娑叉垨鍓嶅垪鑵烘爣鏈腑鐨勮В鑴叉敮鍘熶綋-DNA鍜屾矙鐪艰。鍘熶綋-DNA鍚噺銆傜粨鏋?/span>:涓嶅瓡涓嶈偛鎮ｈ€呰В鑴叉敮鍘熶綋-DNA闃虫€х巼37.78%,娌欑溂琛ｅ師浣?/span>-DNA闃虫€х巼28.89%,瑙ｈ劜鏀師浣撴垨娌欑溂琛ｅ師浣撶殑鎬绘劅鏌撶巼涓?/span>52.22%,姝ｅ父瀵圭収缁勫垯鍒嗗埆涓?/span>15.00%銆?/span>8.33%銆?/span>20.00%,涓ょ粍闂存湁鏄捐憲鎬у樊寮傘€備笉瀛曚笉鑲茬粍涓В鑴叉敮鍘熶綋鐨勬劅鏌撶巼鏄庢樉楂樹簬娌欑溂琛ｅ師浣?/span>(P<0.05)銆傚コ鎬ф偅鑰呰В鑴叉敮鍘熶綋DNA杞介噺鍜屾矙鐪艰。鍘熶綋-DNA杞介噺鍧囬珮浜庣敺鎬ф偅鑰?/span>(P<0.05)銆傜粨璁?/span>:鑽у厜瀹氶噺PCR鎶€鏈敤浜庤瘖鏂紩璧蜂笉瀛曚笉鑲茬殑瑙ｈ劜鏀師浣撴垨娌欑溂琛ｅ師浣撴硨灏跨敓娈栭亾鎰熸煋鏄畬鍏ㄥ彲闈犵殑銆?/span>

7.       闄跺皬鍗?/span>; 閮戠埍濯?/span>; 鑳¤緣; 绛?/span>(鏉窞甯傜鍏汉姘戝尰闄㈡€х梾绉?/span> 310014). 瀹炴椂鑽у厜瀹氶噺鑱氬悎閰堕摼鍙嶅簲妫€娴嬭В鑴茶劜鍘熶綋銆佹矙鐪艰。鍘熶綋鎰熸煋. 涓浗涓タ鍖荤粨鍚堢毊鑲ゆ€х梾瀛︽潅蹇?/span>  2005.06.15; 4(2): 98-99

闄跺皬鍗?/span>绛?/span>閲囩敤瀹炴椂鑽у厜瀹氶噺鑱氬悎閰堕摼鍙嶅簲(FQ-PCR)鎶€鏈?/span>,浠ュ噯纭畾閲忔娴嬪緟妫€鏍囨湰涓В鑴茶劜鍘熶綋(Uu)銆佹矙鐪艰。鍘熶綋(Ct)鐨勬劅鏌撶姸鍐点€傛柟娉?/span>:閲囩敤瀹炴椂FQ-PCR鎶€鏈?/span>,妫€娴嬫爣鏈?/span>650浠?/span>;鐢ㄥ父瑙?/span>PCR鎶€鏈拰瀹炴椂FQ-PCR鎶€鏈?/span>,瀵规瘮妫€娴嬩簡60渚嬨€傜粨鏋?/span>:FQ-PCR妫€娴嬬殑650浠芥爣鏈?/span>,Uu-DNA銆?/span>Ct-DNA妫€鍑虹巼鍒嗗埆涓?/span>24.08%(150/650)銆?/span>16.00%(104/650),鎬绘鍑虹巼涓?/span>36.00%(234/650)銆傚父瑙?/span>PCR缁撴灉閲嶅妫€娴嬫椂,14渚?/span>Uu闃虫€ц€呬腑2渚嬪彉涓洪槾鎬?/span>,鑰?/span>46渚嬮槾鎬ц€呬腑3渚嬪彉涓洪槼鎬?/span>;10渚?/span>Ct闃虫€ц€呬腑1渚嬪彉涓洪槾鎬?/span>,鑰?/span>50渚嬮槾鎬ц€呬腑2渚嬪彉涓洪槼鎬?/span>;FQ-PCR缁撴灉閲嶅妫€娴嬫椂瀹屽叏鍚诲悎銆傜粨璁?/span>:瀹炴椂FQ-PCR鏄娴?/span>Uu銆?/span>Ct涓€涓緝濂界殑鏂规硶銆?/span>

8.         鏋楁晱; 鏉ㄦ(鏌冲窞閾佽矾灞€涓績鍖婚櫌 545007). 瀹炴椂鑽у厜瀹氳仛鍚堥叾閾惧弽搴旀娴嬫硨灏跨敓娈栭亾鐥呭師浣?/span>. 涓崕涓村簥鏉傚織  2003.08.15; 3(4): 49-50

9.         鏉ㄥ噷; 榄忔案绁?/span>; 璧典笟鍗?/span>; (鍖椾含鍚屼粊鍖婚櫌涓績瀹為獙瀹?/span> 100730). 鑽у厜瀹氶噺鑱氬悎閰堕摼娉曡仈鍚堟娴嬫穻鐞冭弻銆佹矙鐪艰。鍘熶綋鍙婅В鑴叉敮鍘熶綋. 涓崕涓村簥鏉傚織  2002.01.30; 2(1): 1-2

10.     鏉ㄥ簡铏?/span>; 鍒樻潹(澶╂触甯備汉姘戝尰闄㈡楠岀, 300132). FQ—PCR妫€娴嬪翱閬撶値鐥呭師浣撶殑涓村簥搴旂敤鍙婅瘎浠?/span>. 涓崕涓村簥鍖诲鐮旂┒鏉傚織  2006; 12(8): 1012-1014

鏉ㄥ簡铏?/span>绛夌爺绌?/span>涓轰复搴婃彁渚涗竴娆″弽搴斿彲鍚屾椂妫€娴?/span>5鐢卞鐟熸皬娣嬬悆鑿?/span>(NG)銆佽В鑴叉敮鍘熶綋(UU)鍙婃矙鐪艰。鍘熶綋(CT)寮曡捣鐨勬穻鑿屾€у翱閬撶値鍜岄潪娣嬭弻鎬у翱閬撶値鐨勮崸鍏夊畾閲?/span>PCR瀹為獙鏂规硶銆傛柟娉?/span>:鍦ㄤ竴娆¤崸鍏夊畾閲?/span>PCR鍙嶅簲涓?/span>,鍒╃敤浠绘剰涓€濂楁爣鍑嗗搧鍚屾椂妫€娴嬩笁绉嶇梾鍘熶綋,杩涜X^2妫€楠屻€傚皢鍚屾牱鐨勬爣鏈垎鍒敤涓夌涓嶅悓璇曞墏鐩掕瘯楠?/span>,灏嗙粨鏋滆繘琛?/span>X^2妫€楠屻€備紭鍖栧弽搴旀潯浠?/span>,姣旇緝缁撴灉鏈夋棤宸紓,杩涜t妫€楠屻€傜粨鏋滃彂鐜颁紭鍖栧弽搴旀潯浠跺悗鏃㈣妭鐪佹椂闂?/span>,鍙堝噺灏戞爣鍑嗗搧鐨勪娇鐢ㄩ噺,涓轰复搴婃彁渚涗簡蹇€熴€佺畝渚裤€佺粡娴庣殑妫€娴嬫柟娉曘€傛瘡绉嶈瘯鍓傜洅閮藉彲妫€娴嬪嚭鐥呭師銆?/span>

11. Welti,-M; Jaton,-K; Altwegg,-M;et al. Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions. Diagn-Microbiol-Infect-Dis. 2003 Feb; 45(2): 85-95

Welti,-M绛?/span>璁捐鐨勫閲嶅疄鏃惰崸鍏夊畾閲?span lang="EN-US">PCR锛屽彲妫€娴嬪懠鍚搁亾鍒嗘硨鐗╀腑鐨勮偤鐐庤。鍘熶綋銆佸棞鑲烘€у啗鍥㈣弻鍜岃偤鐐庢敮鍘熶綋锛屾鏂规硶鏃㈡湁澶氶噸PCR 鍙悓鏃舵娴嬪绉嶇梾鍘熶綋鐨勫姛鑳斤紝鍙堟湁瀹炴椂鑽у厜瀹氶噺PCR 瀹炴椂瀹氶噺鐨勪紭鐐癸紝浣挎娴嬭繃绋嬫洿涓虹畝鍗曘€佺渷鏃讹紝涓斾笌鍚勬敮鍘熶綋鍗曠嫭鐨勫疄鏃惰崸鍏夊畾閲?span lang="EN-US">PCR 妫€娴嬬粨鏋滅浉姣旓紝涓よ€呯殑鐏垫晱搴︽病鏈夋槑鏄惧樊鍒€?/span>

12. Dutro SM, Hebb JK, Garin CA, et al. .Development and performance of a microwell-plate-based polymerase chain reaction assay for Mycoplasma genitalium. Sex Transm Dis. 2003 Oct;30(10):756-63.

Dutro SM绛?/span>鐢?span lang="EN-US">PCR-ELISA 鎶€鏈100 渚嬫硨灏跨敓娈栭亾鏍囨湰涓殑鐢熸畺鏀師浣撹繘琛屾娴嬶紝涓?span lang="EN-US">Southern鏉備氦鎶€鏈叿鏈?span lang="EN-US">{bfb}鐨勪竴鑷寸巼锛屽彲妫€娴嬪埌1-10涓敓娈栨敮鍘熶綋锛岃€屼笉涓庡叾瀹冪浉鍏崇殑寰敓鐗╁弽搴斻€?/span>

13. Mahony JB, Jang D, Chong S, et al.Detection of Chlamydia trachomatis, Neisseria gonorrhoeae, Ureaplasma urealyticum, and Mycoplasma genitalium in First-void Urine Specimens by Multiplex Polymerase Chain Reaction. Mol Diagn. 1997 Sep;2(3):161-168.

Mahony JB绛?/span>璁捐鐨勫閲?span lang="EN-US">PCR 鍙悓鏃舵娴嬪寘鎷劜鍘熶綋銆佺敓娈栨敮鍘熶綋鍦ㄥ唴鐨勫洓绉嶄汉绫绘€т紶鎾柧鐥呭父瑙佺殑鐥呭師浣撱€傚117 渚嬬梾浜虹殑棣栨灏挎爣鏈繘琛屾娴嬶紝鍏舵敮鍘熶綋妫€娴嬬粨鏋滀笌鍗曠嫭鐨?span lang="EN-US">PCR 缁撴灉鍏锋湁{bfb}鐨勪竴鑷寸巼銆?/span>

14. Ferebee,-A; Simoneau,-P; Chang,-J;et al. Differential detection of Mycoplasma pulmonis and Mycoplasma arthritidis with species-specific DNA probes. Diagn-Microbiol-Infect-Dis. 1992 Jul; 15(5): 411-5

Ferebee,-A绛?/span>鎶ラ亾浜嗗簲鐢ㄧ鐗瑰紓鎬х殑DNA鎺㈤拡鏉ラ壌鍒瘖鏂偤鏀師浣撳拰鍏宠妭鐐庢敮鍘熶綋銆?/span>

15. Braddock,-J-A; Tasker,-S; Malik,-R,et al. The use of real-time PCR in the diagnosis and monitoring of Mycoplasma haemofelis copy number in a naturally infected cat. J-Feline-Med-Surg. 2004 Jun; 6(3): 161-5

Braddock,-J-A绛?/span>閫氳繃瀹炴椂鑽у厜瀹氶噺PCR 瀵逛竴鍙弗閲嶈传琛€鐨勭尗杩涜妫€娴嬶紝涓嶄粎鍙戠幇鎰熸煋浜?span lang="EN-US">M. haemofelis锛岃€屼笖鍦ㄦ不鐤楄繃绋嬩腑涔熷彂鐜扮尗浣撳唴鏀師浣?span lang="EN-US">DNA 鏁伴噺鍦ㄩ€愭笎鍑忓皯锛岀洿鑷虫秷澶便€?span lang="EN-US">

16. Kim,-D-J; Park,-J-H; Seok,-S-H;et al. Differential identification of mycoplasma pulmonis and M. arthritidis using PCR-based RFLP. Exp-Anim. 2005 Jul; 54(4): 359-62

   Kim,-D-J绛?/span>鎶ラ亾浜嗗簲鐢?span lang="EN-US"> RFLP-PCR鏉ラ壌鍒瘖鏂偤鏀師浣撳拰鍏宠妭鐐庢敮鍘熶綋銆?span style="mso-bidi-font-size: 10.5pt; mso-hansi-font-family: 瀹嬩綋; mso-bidi-font-weight: bold" lang="EN-US">

浜斻€佹煡鏂板挩璇㈢粨璁?/span>

鏍规嵁鐢宠浜烘煡鏂拌姹傦紝鎻愪緵鐨勬煡鏂板唴瀹癸紝妫€绱㈢敤璇嶄互鍙婄敤涓婅堪妫€绱㈢瓥鐣ワ紝缁忔绱?span lang="EN-US">CBM锛?span lang="EN-US">DISC銆?/span>CHKD璺ㄥ簱妫€绱㈠钩鍙般€佸浗瀹剁鎶€鎴愭灉搴撱€?/span>鍥藉绉戞妧鍥句功鏂囩尞涓績銆?/span>MEDLINE-PUBMED浜斾釜妫€绱㈠伐鍏?span lang="EN-US">/绯荤粺锛岃秴杩?span lang="EN-US">10骞存椂闂达紝澶х害4970涓囩瘒鏂囩尞锛屾湭瑙佹湁鍏冲缓绔嬪悓鏃舵娴嬪绉嶆敮鍘熶綋澶氶噸鑽у厜瀹氶噺PCR娉曠殑鐩稿叧鏂囩尞锛?/span>鍏蜂綋鍒嗘瀽濡備笅锛?o:p>

1銆?span style="FONT: 7pt "Times New Roman"">   鎺㈣鍚屾椂妫€娴嬪绉嶇梾鍘熶綋鏂规硶鐨勭浉鍏虫枃鐚?o:p>

鍥藉唴鐩稿叧鏂囩尞锛氬寳浜ぇ瀛︿复搴婅偪鐦ゅ闄㈠寳浜競鑲跨槫闃叉不鐮旂┒鏉ㄦ枃鍚?/span>绛?/span>鍒跺浜嗛拡瀵逛复搴婂父瑙佺殑鍏鏀師浣撶殑鐗瑰紓鎬у崟鍏嬮殕鎶椾綋,涓轰复搴婃敮鍘熶綋鎰熸煋妫€娴嬫彁渚涘伐鍏枫€傜敤鐏椿鐨勬敮鍘熶綋棰楃矑鍏嶇柅BALB/c灏忛紶,閫氳繃浼犵粺鏉備氦鐦ゆ妧鏈埗澶囨姉鏀師浣撳崟鍏嬮殕鎶椾綋,閲囩敤6绉嶆敮鍘熶綋铔嬬櫧浠ラ叾鑱斿厤鐤惛闄勮瘯楠?/span>(ELISA)杩涜浜ゅ弶绛涢€?/span>,骞剁敤鍏嶇柅鍗拌娉?/span>(WB)瀵瑰叾鐗瑰紓鎬ц繘琛岄壌瀹氥€傜粨鏋滆幏寰椾簡閽堝6绉嶄汉浣撳父瑙佹劅鏌撴敮鍘熶綋鐨勪笉鍚屽崟鍏嬮殕鎶椾綋,瀹冧滑鍦?/span>ELISA妫€娴?/span>涓叿杈冨ソ鐨勫弽搴旂壒寮傛€?span lang="EN-US">,鍙簲鐢ㄤ簬鏀師浣撴劅鏌撴娴?/span>锛堟枃鐚?span lang="EN-US">1锛夈€?/span>姝︽眽甯傜涓€鍖婚櫌鐨偆绉戠帇杈?/span>绛?/span>璇勪环浜嗗弽鍚戠嚎鐐规潅浜ゆ柟娉曟娴嬬敓娈栨敮鍘熶綋銆佷汉鍨嬫敮鍘熶綋銆佸井灏忚劜鍘熶綋鍜岃В鑴茶劜鍘熶綋鐨勬晱鎰熸€с€傚簲鐢ㄦ敮鍘熶綋鍙嶅悜绾跨偣鏉備氦鏂规硶鍜岀鐗瑰紓鎬?/span>PCR鏂规硶鍚屾椂妫€娴?/span>198渚嬩复搴婃爣鏈?/span>,姣旇緝涓ょ鏂规硶鐨勬娴嬬粨鏋溿€傜粨鏋滄樉绀哄弽鍚戠嚎鐐规潅浜ゆ柟娉曡兘鍚屾椂蹇€熴€佹晱鎰熷拰鐗瑰紓鐨勬娴嬭繖4绉嶆敮鍘熶綋锛堟枃鐚?span lang="EN-US">2锛夈€?/span>鏃犻敗甯傚厠闅嗛仐浼犳妧鏈爺绌舵墍绯滅鐓?/span>绛?/span>搴旂敤鍙岄噸濂楀紡PCR鎶€鏈悓鏃舵娴嬪彂閰垫敮鍘熶綋(Mf)鍜岀┛閫氭敮鍘熶綋(Mpe)銆備互鏀師浣?/span>16SvRNA鍩哄洜涓洪澏鍩哄洜,澶栧寮曠墿涓?/span>Mf涓?/span>Mpe鍏辩敤銆佸唴濂楀紩鐗╁垯鍒嗗埆涓?/span>Mf绉嶇壒寮傚拰Mpe绉嶇壒寮?/span>,寤虹珛鍙岄噸濂楀紡鎵╁浣撶郴銆傜粨鏋滄樉绀?/span>鍙岄噸濂楀紡PCR鏄竴绉嶇伒鏁忋€佺壒寮傚拰蹇€熺殑Mf鍜?span lang="EN-US">Mpe妫€娴嬫柟娉?/span>锛堟枃鐚?span lang="EN-US">3锛夈€?span lang="EN-US">

鍥藉鐩稿叧鏂囩尞锛?/span>Welti,-M绛?/span>璁捐鐨勫閲嶅疄鏃惰崸鍏夊畾閲?span lang="EN-US">PCR锛屽彲妫€娴嬪懠鍚搁亾鍒嗘硨鐗╀腑鐨勮偤鐐庤。鍘熶綋銆佸棞鑲烘€у啗鍥㈣弻鍜岃偤鐐庢敮鍘熶綋锛屾鏂规硶鏃㈡湁澶氶噸PCR 鍙悓鏃舵娴嬪绉嶇梾鍘熶綋鐨勫姛鑳斤紝鍙堟湁瀹炴椂鑽у厜瀹氶噺PCR 瀹炴椂瀹氶噺鐨勪紭鐐?/span>锛堟枃鐚?span lang="EN-US">11锛夈€?/span>Dutro SM绛?/span>鐢?span lang="EN-US">PCR-ELISA 鎶€鏈100 渚嬫硨灏跨敓娈栭亾鏍囨湰涓殑鐢熸畺鏀師浣撹繘琛屾娴嬶紝涓?span lang="EN-US">Southern鏉備氦鎶€鏈叿鏈?span lang="EN-US">{bfb}鐨勪竴鑷寸巼锛屽彲妫€娴嬪埌1-10涓敓娈栨敮鍘熶綋锛岃€屼笉涓庡叾瀹冪浉鍏崇殑寰敓鐗╁弽搴?/span>锛堟枃鐚?2锛夈€?/span>Mahony JB绛?/span>璁捐鐨勫閲?span lang="EN-US">PCR 鍙悓鏃舵娴嬪寘鎷劜鍘熶綋銆佺敓娈栨敮鍘熶綋鍦ㄥ唴鐨勫洓绉嶄汉绫绘€т紶鎾柧鐥呭父瑙佺殑鐥呭師浣?/span>锛堟枃鐚?span lang="EN-US">13锛夈€?/span>

2銆佹帰璁?span style="COLOR: black">鑽у厜瀹氶噺PCR鏂规硶妫€娴嬫敮鍘熶綋鐨勭浉鍏虫枃鐚?/span>

鍥藉唴鐩稿叧鏂囩尞锛氭繁鍦冲競绂忔案鍖婚櫌鍚戝崕鍥界瓑鐮旂┒寤虹珛鍜岃瘎浠蜂竴涓柊鐨勫閲?/span>PCR.鍙嶅悜绾跨偣鏉備氦鎶€鏈?/span>(RIJB)蹇€熷悓鏃舵娴嬫硨灏跨敓娈栭亾娌欑溂琛ｅ師浣?/span>(Ct)銆佹穻鐥呭鐟熻弻(鍧?/span>)鍜?/span>3绉嶆敮鍘熶綋鎰熸煋鐨勬柟娉曘€傚垎鍒€夋嫨Ct闅愯斀璐ㄧ矑鍜?/span>Ng16SrRNA鍩哄洜璁捐涓ゅ鐗瑰紓鎬у紩鐗?/span>,浠ユ敮鍘熶綋鍐呰浆褰曢棿闅斿簭鍒?/span>(ITS)璁捐涓€瀵规敮鍘熶綋灞為€氱敤寮曠墿,鐢熺墿绱犳爣璁颁笅娓稿紩鐗┿€傛瀯寤轰笁閲?/span>PCR鍚屾椂鎵╁Ct銆?/span>Ng銆佽В鑴茶劜鍘熶綋銆佸井灏忚劜鍘熶綋銆佷汉鍨嬫敮鍘熶綋(Mh)绛夎弻DNA,鐒跺悗涓庡浐瀹氬湪灏奸緳鑶滀笂鐨勫悇鐗瑰紓鎬у鏍歌嫹閰告帰閽堟潅浜ゃ€傚苟瀵?/span>142浠界粡鑽у厜瀹氶噺PCR(FQ-PCR)妫€娴?/span>0鍜屽潧鐨勬€х梾楂樺嵄浜虹兢鏍囨湰,浠ュ強45浠界粡鏀師浣撴恫浣撳煿鍏绘硶閴村畾鐨勬爣鏈繘琛屾娴嬨€傜粨鏋滃彂鐜板閲?/span>PCR-RLB鍙揩閫熷悓鏃舵娴?/span>Ct銆?/span>Ng銆?/span>Uu銆?/span>Up鍜?/span>Mh,涓烘€т紶鎾柧鐥呯殑涓村簥璇婃柇鎻愪緵浜嗕竴绉嶅彲闈犵殑鏂规硶锛堟枃鐚?span lang="EN-US">4锛夈€?/span>娌堥槼涓浗鍖荤澶у绗簩涓村簥瀛﹂櫌妫€楠岀瀛欑户姊?/span>绛?/span>鐢ㄨ崸鍏夊畾閲忚仛鍚堥叾閾惧弽搴?/span>(FQ-PCR)妫€娴嬫硨灏跨敓娈栭亾鎴栧棰堝垎娉岀墿涓穻鐞冭弻(NG)銆佹矙鐪艰。鍘熶綋(CT)銆佽В鑴叉敮鍘熶綋(UU)鐨勬暟閲?/span>,浠ヤ簡瑙ｄ笂杩?/span>3绉嶇梾鍘熶綋鎰熸煋鐜扮姸銆傜粨鏋滃彂鐜板簲鐢?/span>FQ-PCR鍏锋湁绠€渚裤€佸揩閫熴€佸噯纭殑浼樼偣,1浠芥爣鏈彲鍚屾椂妫€娴?/span>3绉嶇梾鍘熶綋,鏄洰鍓嶅揩閫熻瘖鏂穻鐥?/span>,闈炴穻鑿屾€у翱閬撶値鍙潬鍑嗙‘鐨勬柟娉?/span>锛堟枃鐚?span lang="EN-US">5锛夈€?/span>鐢樿們鐪佸钩鍑夊競浜烘皯鍖婚櫌浠囪緟鍚涢噰鐢ㄥ疄鏃惰崸鍏夊畾閲?/span>PCR 鎶€鏈娴?/span>45瀵逛笉瀛曚笉鑲插か濡囧コ鎬у棰堝垎娉岀墿銆佺敺鎬х簿娑叉垨鍓嶅垪鑵烘爣鏈腑鐨勮В鑴叉敮鍘熶綋-DNA鍜屾矙鐪艰。鍘熶綋-DNA鍚噺銆傜粨鏋滄樉绀鸿崸鍏夊畾閲?/span>PCR鎶€鏈敤浜庤瘖鏂紩璧蜂笉瀛曚笉鑲茬殑瑙ｈ劜鏀師浣撴垨娌欑溂琛ｅ師浣撴硨灏跨敓娈栭亾鎰熸煋鏄畬鍏ㄥ彲闈犵殑锛堟枃鐚?span lang="EN-US">6锛夈€?/span>鏉窞甯傜鍏汉姘戝尰闄㈡€х梾绉戦櫠灏忓崕绛?/span>閲囩敤瀹炴椂FQ-PCR鎶€鏈?/span>,妫€娴嬫爣鏈?/span>650浠?/span>;鐢ㄥ父瑙?/span>PCR鎶€鏈拰瀹炴椂FQ-PCR鎶€鏈?/span>,瀵规瘮妫€娴嬩簡60渚嬨€傜粨鏋滃彂鐜板疄鏃?/span>FQ-PCR鏄娴?/span>Uu銆?/span>Ct涓€涓緝濂界殑鏂规硶锛堟枃鐚?span lang="EN-US">7锛夈€?/span>澶╂触甯備汉姘戝尰闄㈡楠岀鏉ㄥ簡铏?/span>绛?/span>鍦ㄤ竴娆¤崸鍏夊畾閲?/span>PCR鍙嶅簲涓?/span>,鍒╃敤浠绘剰涓€濂楁爣鍑嗗搧鍚屾椂妫€娴嬩笁绉嶇梾鍘熶綋,杩涜X^2妫€楠屻€傚皢鍚屾牱鐨勬爣鏈垎鍒敤涓夌涓嶅悓璇曞墏鐩掕瘯楠?/span>,灏嗙粨鏋滆繘琛?/span>X^2妫€楠屻€備紭鍖栧弽搴旀潯浠?/span>,姣旇緝缁撴灉鏈夋棤宸紓,杩涜t妫€楠屻€傜粨鏋滃彂鐜颁紭鍖栧弽搴旀潯浠跺悗鏃㈣妭鐪佹椂闂?/span>,鍙堝噺灏戞爣鍑嗗搧鐨勪娇鐢ㄩ噺,涓轰复搴婃彁渚涗簡蹇€熴€佺畝渚裤€佺粡娴庣殑妫€娴嬫柟娉?/span>锛堟枃鐚?span lang="EN-US">10锛夈€?span lang="EN-US">

鍥藉鐩稿叧鏂囩尞锛?/span>Welti,-M绛?/span>璁捐鐨勫閲嶅疄鏃惰崸鍏夊畾閲?span lang="EN-US">PCR锛屽彲妫€娴嬪懠鍚搁亾鍒嗘硨鐗╀腑鐨勮偤鐐庤。鍘熶綋銆佸棞鑲烘€у啗鍥㈣弻鍜岃偤鐐庢敮鍘熶綋锛堟枃鐚?span lang="EN-US">11锛夈€?/span>Braddock,-J-A绛?/span>閫氳繃瀹炴椂鑽у厜瀹氶噺PCR 瀵逛竴鍙弗閲嶈传琛€鐨勭尗杩涜妫€娴嬶紝涓嶄粎鍙戠幇鎰熸煋浜?span lang="EN-US">M. haemofelis锛岃€屼笖鍦ㄦ不鐤楄繃绋嬩腑涔熷彂鐜扮尗浣撳唴鏀師浣?span lang="EN-US">DNA 鏁伴噺鍦ㄩ€愭笎鍑忓皯锛岀洿鑷虫秷澶?/span>锛堟枃鐚?5锛夈€?/span>Kim,-D-J绛?/span>鎶ラ亾浜嗗簲鐢?/span> RFLP-PCR鏉ラ壌鍒瘖鏂偤鏀師浣撳拰鍏宠妭鐐庢敮鍘熶綋锛堟枃鐚?6锛夈€?/span>

缁间笂鎵€杩帮紝涓庡浗鍐呭鏂囩尞瀵规瘮鍒嗘瀽缁撹濡備笅锛?o:p>

1銆佸浗鍐?/span>鍙鐗瑰紓鎬у崟鍏嬮殕鎶椾綋妫€娴?/span>涓村簥甯歌鐨勫叚绉嶆敮鍘熶綋鐨?/span>鏂囩尞鎶ラ亾锛堣鏂囩尞1锛夛紝涔熷彲瑙?/span>鍙嶅悜绾跨偣鏉備氦鏂规硶銆佸弻閲嶅寮?/span>PCR妫€娴嬪绉嶆敮鍘熶綋鐨勬枃鐚姤閬擄紙瑙佹枃鐚?/span>2锛?/span>3锛夛紝浣嗘湭瑙佽繍鐢ㄥ閲嶅疄鏃惰崸鍏夊畾閲?/span>PCR鎶€鏈悓鏃舵娴嬪绉嶆敮鍘熶綋鐨勬枃鐚姤閬?/span>銆傚浗澶栦篃鏈杩愮敤澶氶噸瀹炴椂鑽у厜瀹氶噺PCR鎶€鏈悓鏃舵娴嬪绉嶆敮鍘熶綋鐨勬枃鐚姤閬撱€?/span>

2銆?/span>鍥藉唴鍙澶氶噸PCR鎴栧疄鏃惰崸鍏夊畾閲?/span>PCR鎶€鏈娴嬫敮鍘熶綋鐨勭爺绌舵枃鐚姤閬擄紙瑙佹枃鐚?span lang="EN-US">4-10锛夛紝浣嗘湭瑙?/span>杩愮敤澶氶噸瀹炴椂鑽у厜瀹氶噺PCR鎶€鏈悓鏃舵娴嬫敮鍘?/span>浣撳拰鑲烘敮鍘熶綋銆佸叧鑺傜値鏀師浣撱€佹憾绁炵粡鏀師浣?/span>鐨勭爺绌舵枃鐚姤閬撱€?/span>鍥藉鍙澶氶噸瀹炴椂鑽у厜瀹氶噺PCR妫€娴嬪绉嶇梾鍘熶綋鐨勭爺绌舵枃鐚姤閬擄紙瑙佹枃鐚?span lang="EN-US">11锛夛紝浣嗘湭瑙?/span>杩愮敤澶氶噸瀹炴椂鑽у厜瀹氶噺PCR鎶€鏈悓鏃舵娴嬫敮鍘?/span>浣撳拰鑲烘敮鍘熶綋銆佸叧鑺傜値鏀師浣撱€佹憾绁炵粡鏀師浣?/span>鐨勭爺绌舵枃鐚姤閬撱€?/span>