7.硼砂-氢氧化钠缓冲液（0.05M硼酸根）
X ml 0.05M硼砂+Y ml 0.2M氢氧化钠，再加水稀释至200ml。
| pH | X/ml | Y/ml | pH | X/ml | Y/ml |
| 9.3 | 50 | 6.0 | 9.8 | 50 | 34.0 |
| 9.4 | 50 | 11.0 | 10.0 | 50 | 43.0 |
| 9.6 | 50 | 23.0 | 10.1 | 50 | 46.0 |
硼砂Na2B4O7·10H2O分子量=381.43
0.05M(=0.2M硼酸根)溶液为19.07g/L
8.巴比妥钠-盐酸缓冲液（18℃）
| pH | 0.04M巴比妥钠/ml | 0.2M盐酸/ml | pH | 0.04M巴比妥钠/ml | 0.2M盐酸/ml |
| 6.8 | 100 | 18.4 | 8.4 | 100 | 5.21 |
| 7.0 | 100 | 17.8 | 8.6 | 100 | 3.82 |
| 7.2 | 100 | 16.7 | 8.8 | 100 | 2.52 |
| 7.4 | 100 | 15.3 | 9.0 | 100 | 1.65 |
| 7.6 | 100 | 13.4 | 9.2 | 100 | 1.13 |
| 7.8 | 100 | 11.47 | 9.4 | 100 | 0.70 |
| 8.0 | 100 | 9.39 | 9.6 | 100 | 0.35 |
| 8.2 | 100 | 7.21 |
9.Tris-盐酸缓冲液
50ml 0.1M三羟甲基氨基甲烷(Tris)溶液于X ml 0.1M盐酸混匀后，加水稀释至100ml。
| pH | X/ml | pH | X/ml |
| 7.1 | 45.7 | 8.1 | 26.2 |
| 7.2 | 44.7 | 8.2 | 22.9 |
| 7.3 | 43.4 | 8.3 | 19.9 |
| 7.4 | 42.0 | 8.4 | 17.2 |
| 7.5 | 40.3 | 8.5 | 14.7 |
| 7.6 | 38.5 | 8.6 | 12.4 |
| 7.7 | 36.6 | 8.7 | 10.3 |
| 7.8 | 34.5 | 8.8 | 8.5 |
| 7.9 | 32.0 | 8.9 | 7.0 |
| 8.0 | 29.2 |
三羟甲基氨基甲烷(Tris)分子量=121.14 0.1M溶液为12.114g/L
Tris溶液可以从空气中吸收二氧化碳，使用时注意将瓶盖严。 ww
10.硼酸-硼砂缓冲液（0.2M硼酸根）
| pH | 0.05M硼砂/ml | 0.2M硼酸/ml | pH | 0.05M硼砂/ml | 0.2M硼酸/ml |
| 7.4 | 1.0 | 9.0 | 8.2 | 3.5 | 6.5 |
| 7.6 | 1.5 | 8.5 | 8.4 | 4.5 | 5.5 |
| 7.8 | 2.0 | 8.0 | 8.7 | 6.0 | 4.0 |
| 8.0 | 3.0 | 7.0 | 9.0 | 8.0 | 2.0 |
硼砂Na2B4O7·10H2O分子量=381.43 0.05M(=0.2M硼酸根)溶液为19.07g/L
硼酸H3BO3分子量=61.84 0.2M溶液为12.37g/L
硼砂易失去结晶水，必须在带塞的瓶中保存
11.甘氨酸-氢氧化钠缓冲液（0.05M）
X ml 0.2M甘氨酸+Y ml 0.2M氢氧化钠，再加水稀释至200ml。
| pH | X/ml | Y/ml | pH | X/ml | Y/ml |
| 8.6 | 50 | 4.0 | 9.6 | 50 | 22.4 |
| 8.8 | 50 | 6.0 | 9.8 | 50 | 27.2 |
| 9.0 | 50 | 8.8 | 10.0 | 50 | 32.0 |
| 9.2 | 50 | 12.0 | 10.4 | 50 | 38.6 |
| 9.4 | 50 | 16.8 | 10.6 | 50 | 45.5 |
甘氨酸分子量=75.07 0.2M甘氨酸溶液含15.01g/L
