PCR 寮曠墿璁捐鍙奝remier Primer 銆丱ligo 杞欢浣跨敤鎶€宸э紙甯﹀浘鐗囷級Time锛?009-04-11 PM 21:45銆€銆€Author:bioer銆€銆€Hits:銆€锛堜腑鍥藉崗鍜屽尰绉戝ぇ瀛︿腑鍥藉尰瀛︾瀛﹂櫌鑲跨槫鐮旂┒鎵€锛屽寳浜?00021锛?br />        鎽樿锛氭湰鏂囨棬鍦ㄤ粙缁嶄娇鐢ㄨ蒋浠惰璁CR 寮曠墿鐨勬妧宸с€傚湪PCR 寮曠墿璁捐鍘熷垯鐨勫熀纭€涓婏紝璇︾粏浠嬬粛浜嗕袱绉嶅父鐢ㄥ紩鐗╄璁¤蒋浠剁殑鍩烘湰浣跨敤鏂规硶锛屽苟瀵瑰叾鍚勮嚜鐨勪紭缂虹偣杩涜浜嗘瘮杈冦€備竴鑸€у紩鐗╄嚜鍔ㄦ悳绱㈠彲閲囩敤“Premier Primer 5”杞欢锛岃€屽紩鐗╃殑璇勪环鍒嗘瀽鍒欏彲閲囩敤“Oligo 6”杞欢銆?br />鍏抽敭璇嶏細PCR锛涘紩鐗╄璁?br />涓浘鍒嗙被鍙凤細Q524 鏂囩尞鏍囪瘑鐮侊細 鏂囩珷缂栧彿锛?/p>

         鑷粠1985 骞碖arny Mullis 鍙戞槑浜嗚仛鍚堥叾閾惧紡鍙嶅簲浠ユ潵锛孭CR 鎶€鏈凡鎴愪负鍒嗗瓙鐢熺墿瀛︾爺绌朵腑浣跨敤鏈€澶氥€佹渶骞挎硾鐨勬墜娈典箣涓€锛岃€屽紩鐗╄璁℃槸PCR 鎶€鏈腑鑷冲叧閲嶈鐨勪竴鐜€備娇鐢ㄤ笉鍚堥€傜殑PCR 寮曠墿瀹规槗瀵艰嚧瀹為獙澶辫触锛氳〃鐜颁负鎵╁鍑虹洰鐨勫甫涔嬪鐨勫鏉″甫锛堝褰㈡垚寮曠墿浜岃仛浣撳甫锛夛紝涓嶅嚭甯︽垨鍑哄甫寰堝急锛岀瓑绛夈€傜幇鍦≒CR 寮曠墿璁捐澶ч兘閫氳繃璁＄畻鏈鸿蒋浠惰繘琛屻€傚彲浠ョ洿鎺ユ彁浜ゆā鏉垮簭鍒楀埌鐗瑰畾缃戦〉锛屽緱鍒拌璁″ソ鐨勫紩鐗╋紝涔熷彲浠ュ湪鏈湴璁＄畻鏈轰笂杩愯寮曠墿璁捐涓撲笟杞欢銆備竴鑸潵璇达紝涓撻棬杩涜PCR寮曠墿璁捐鐨勪笓涓氳蒋浠跺姛鑳芥洿涓哄己澶э紝浣嗕娇鐢ㄨ捣鏉ュ嵈涓嶅お瀹规槗銆傛湰鏂囧皢灏卞紩鐗╄璁″師鍒欏強杞欢浣跨敤闂杩涜鎺㈣銆?br />寮曠墿璁捐鐨勫師鍒?br />        寮曠墿璁捐鏈? 鏉″熀鏈師鍒欙細棣栧厛寮曠墿涓庢ā鏉跨殑搴忓垪瑕佺揣瀵嗕簰琛ワ紝鍏舵寮曠墿涓庡紩鐗╀箣闂撮伩鍏嶅舰鎴愮ǔ瀹氱殑浜岃仛浣撴垨鍙戝す缁撴瀯锛屽啀娆″紩鐗╀笉鑳藉湪妯℃澘鐨勯潪鐩殑浣嶇偣寮曞彂DNA 鑱氬悎鍙嶅簲(鍗抽敊閰?銆?br />        鍏蜂綋瀹炵幇杩? 鏉″熀鏈師鍒欓渶瑕佽€冭檻鍒拌澶氬洜绱狅紝濡傚紩鐗╅暱搴︼紙primer length锛夛紝浜х墿闀垮害锛坧roduct length锛夛紝搴忓垪Tm 鍊?melting temperature)锛屽紩鐗╀笌妯℃澘褰㈡垚鍙岄摼鐨勫唴閮ㄧǔ瀹氭€?internal stability, 鐢?G 鍊煎弽鏄?锛屽舰鎴愬紩鐗╀簩鑱氫綋(primer dimer)鍙婂彂澶圭粨鏋勶紙duplex formation and hairpin锛夌殑鑳藉€硷紝鍦ㄩ敊閰嶄綅鐐癸紙false priming site锛夌殑寮曞彂鏁堢巼锛屽紩鐗╁強浜х墿鐨凣C 鍚噺锛坈omposition锛夛紝绛夌瓑銆傚繀瑕佹椂杩橀渶瀵瑰紩鐗╄繘琛屼慨楗帮紝濡傚鍔犻檺鍒舵€у唴鍒囬叾浣嶇偣锛屽紩杩涚獊鍙樼瓑銆傛牴鎹湁鍏冲弬鑰冭祫鏂欏拰绗旇€呭湪瀹炶返涓殑鎬荤粨锛屽紩鐗╄璁″簲娉ㄦ剰濡備笅瑕佺偣锛?br />1. 寮曠墿鐨勯暱搴︿竴鑸负15-30 bp锛屽父鐢ㄧ殑鏄?8-27 bp锛屼絾涓嶅簲澶т簬38锛屽洜涓鸿繃闀夸細瀵艰嚧鍏跺欢浼告俯搴﹀ぇ浜?4鈩冿紝涓嶉€備簬Taq DNA 鑱氬悎閰惰繘琛屽弽搴斻€?br />2. 寮曠墿搴忓垪鍦ㄦā鏉垮唴搴斿綋娌℃湁鐩镐技鎬ц緝楂橈紝灏ゅ叾鏄?’绔浉浼兼€ц緝楂樼殑搴忓垪锛屽惁鍒欏鏄撳鑷撮敊閰嶃€傚紩鐗?’绔嚭鐜? 涓互涓婄殑杩炵画纰卞熀锛屽GGG 鎴朇CC锛屼篃浼氫娇閿欒寮曞彂鏈虹巼澧炲姞銆?br />3. 寮曠墿3’绔殑鏈綅纰卞熀瀵筎aq 閰剁殑DNA 鍚堟垚鏁堢巼鏈夎緝澶х殑褰卞搷銆備笉鍚岀殑鏈綅纰卞熀鍦ㄩ敊閰嶄綅缃鑷翠笉鍚岀殑鎵╁鏁堢巼锛屾湯浣嶇⒈鍩轰负A 鐨勯敊閰嶆晥鐜囨槑鏄鹃珮浜庡叾浠? 涓⒈鍩猴紝鍥犳搴斿綋閬垮厤鍦ㄥ紩鐗╃殑3’绔娇鐢ㄧ⒈鍩篈銆傚彟澶栵紝寮曠墿浜岃仛浣撴垨鍙戝す缁撴瀯涔熷彲鑳藉鑷碢CR 鍙嶅簲澶辫触銆?’绔簭鍒楀PCR 褰卞搷涓嶅お澶э紝鍥犳甯哥敤鏉ュ紩杩涗慨楗颁綅鐐规垨鏍囪鐗┿€?br />4. 寮曠墿搴忓垪鐨凣C 鍚噺涓€鑸负40-60%锛岃繃楂樻垨杩囦綆閮戒笉鍒╀簬寮曞彂鍙嶅簲銆備笂涓嬫父寮曠墿鐨凣C鍚噺涓嶈兘鐩稿樊澶ぇ銆?br />5. 寮曠墿鎵€瀵瑰簲妯℃澘浣嶇疆搴忓垪鐨凾m 鍊煎湪72鈩冨乏鍙冲彲浣垮鎬ф潯浠舵渶浣炽€俆m 鍊肩殑璁＄畻鏈夊绉嶆柟娉曪紝濡傛寜鍏紡Tm锛?(G+C)锛?(A+T)锛屽湪Oligo 杞欢涓娇鐢ㄧ殑鏄渶閭昏繎娉?the nearest neighbor method) 銆?br />6. ?G 鍊兼槸鎸嘍NA 鍙岄摼褰㈡垚鎵€闇€鐨勮嚜鐢辫兘锛岃鍊煎弽鏄犱簡鍙岄摼缁撴瀯鍐呴儴纰卞熀瀵圭殑鐩稿绋冲畾鎬с€傚簲褰撻€夌敤3’绔?G 鍊艰緝浣庯紙缁濆鍊间笉瓒呰繃9锛夛紝鑰?’绔拰涓棿?G 鍊肩浉瀵硅緝楂樼殑寮曠墿銆傚紩鐗╃殑3’绔殑?G 鍊艰繃楂橈紝瀹规槗鍦ㄩ敊閰嶄綅鐐瑰舰鎴愬弻閾剧粨鏋勫苟寮曞彂DNA 鑱氬悎鍙嶅簲[6]銆?br />7. 寮曠墿浜岃仛浣撳強鍙戝す缁撴瀯鐨勮兘鍊艰繃楂橈紙瓒呰繃4.5kcal/mol锛夋槗瀵艰嚧浜х敓寮曠墿浜岃仛浣撳甫锛屽苟涓旈檷浣庡紩鐗╂湁鏁堟祿搴﹁€屼娇PCR 鍙嶅簲涓嶈兘姝ｅ父杩涜銆?br />8. 瀵瑰紩鐗╃殑淇グ涓€鑸槸鍦?’绔鍔犻叾鍒囦綅鐐癸紝搴旀牴鎹笅涓€姝ュ疄楠屼腑瑕佹彃鍏CR 浜х墿鐨勮浇浣撶殑鐩稿簲搴忓垪鑰岀‘瀹氥€?br />        鍊煎緱涓€鎻愮殑鏄紝鍚勭妯℃澘鐨勫紩鐗╄璁￠毦搴︿笉涓€銆傛湁鐨勬ā鏉挎湰韬潯浠舵瘮杈冨洶闅撅紝渚嬪GC鍚噺鍋忛珮鎴栧亸浣庯紝瀵艰嚧鎵句笉鍒板悇绉嶆寚鏍囬兘鍗佸垎鍚堥€傜殑寮曠墿锛涘湪鐢ㄤ綔鍏嬮殕鐩殑鐨凱CR 鍥犱负浜х墿搴忓垪鐩稿鍥哄畾锛屽紩鐗╄璁＄殑閫夋嫨鑷敱搴﹁緝浣庛€傚湪杩欑鎯呭喌鍙兘閫€鑰屾眰鍏舵锛屽敖閲忓幓婊¤冻鏉′欢銆?br />寮曠墿鐨勮嚜鍔ㄦ悳绱㈠拰璇勪环鍒嗘瀽
        杞欢鐨勫紩鐗╄璁″姛鑳戒富瑕佷綋鐜板湪涓や釜鏂归潰锛氶鍏堟槸寮曠墿鍒嗘瀽璇勪环鍔熻兘锛岃鍔熻兘鍙湁灏戞暟鍟嗕笟鐗堣蒋浠惰兘澶熷仛鍒帮紝鍏朵腑浠?ldquo;Oligo 6”鏈€浼樼锛涘叾娆℃槸寮曠墿鐨勮嚜鍔ㄦ悳绱㈠姛鑳斤紝鍚勭杞欢鍦ㄨ繖鏂归潰鐨勪晶閲嶇偣涓嶅悓锛屽洜姝よ嚜鍔ㄦ悳绱㈢殑缁撴灉涔熶笉灏界浉鍚屻€傛嵁绗旇€呯殑缁忛獙锛岃嚜鍔ㄦ悳绱㈠姛鑳戒互“Premier Primer”涓烘渶寮轰笖鏂逛究浣跨敤锛?ldquo;Oligo 6”鍏舵锛屽叾浠栬蒋浠跺“Vector NTI Suit”銆?ldquo;Dnasis”銆?ldquo;Omiga”鍜?ldquo;Dnastar”閮藉甫鏈夊紩鐗╄嚜鍔ㄦ悳绱㈠姛鑳斤紝浣嗘悳绱㈢粨鏋滀笉鏄崄鍒嗙悊鎯炽€傝鎯冲緱鍒版晥鏋滃緢濂界殑寮曠墿锛屽湪鑷姩鎼滅储鐨勫熀纭€涓婅繕瑕佽緟浠ヤ汉宸ュ垎鏋愩€傜瑪鑰呰涓哄紩鐗╄璁¤蒋浠剁殑鏈€浣虫惌閰嶆槸“Oligo”鍜?ldquo;Premier”杞欢鍚堝苟浣跨敤锛屼互“Premier”杩涜鑷姩鎼滅储锛?ldquo;Oligo”杩涜鍒嗘瀽璇勪环锛屽姝ゅ彲蹇€熻璁″嚭鎴愬姛鐜囧緢楂樼殑寮曠墿銆?br />Primer Premier 5.0 鐨勪娇鐢ㄦ妧宸х畝浠?br />1. 鍔熻兘
        “Premier”鐨勪富瑕佸姛鑳藉垎鍥涘ぇ鍧楋紝鍏朵腑鏈変笁绉嶅姛鑳芥瘮杈冨父鐢紝鍗冲紩鐗╄璁°€侀檺鍒舵€у唴鍒囬叾浣嶇偣鍒嗘瀽鍜孌NA 鍩哄厓(motif)鏌ユ壘銆?ldquo;Premier”杩樺叿鏈夊悓婧愭€у垎鏋愬姛鑳斤紝浣嗗苟闈炲叾鐗归暱锛屽湪姝ょ暐杩囥€傛澶栵紝璇ヨ蒋浠惰繕鏈変竴浜涚壒娈婂姛鑳斤紝鍏朵腑鏈€閲嶈鐨勬槸璁捐绠€骞跺紩鐗╋紝鍙﹀杩樻湁搴忓垪“鏈楄”銆丏NA 涓庤泲鐧藉簭鍒楃殑浜掓崲銆佽闊虫彁绀洪敭鐩樿緭鍏ョ瓑绛夈€?br />         鏈夋椂闇€瑕佹牴鎹竴娈垫皑鍩洪吀搴忓垪鍙嶆帹鍒癉NA 鏉ヨ璁″紩鐗╋紝鐢变簬澶у鏁版皑鍩洪吀锛?0 绉嶅父瑙佺粨鏋勬皑鍩洪吀涓殑18 绉嶏級鐨勯仐浼犲瘑鐮佷笉鍙竴绉嶏紝鍥犳锛岀敱姘ㄥ熀閰稿簭鍒楀弽鎺―NA 搴忓垪鏃讹紝浼氶亣鍒伴儴鍒嗙⒈鍩虹殑涓嶇‘瀹氭€с€傝繖鏍疯璁″苟鍚堟垚鐨勫紩鐗╁疄闄呬笂鏄涓簭鍒楃殑娣峰拰鐗╋紝瀹冧滑鐨勫簭鍒楃粍鎴愬ぇ閮ㄥ垎鐩稿悓锛屼絾鍦ㄦ煇浜涗綅鐐规湁鎵€鍙樺寲锛岀О涔嬩负绠€骞跺紩鐗┿€傞仐浼犲瘑鐮佽鍒欏洜鐗╃鎴栫粏鑳炰簹缁撴瀯鐨勪笉鍚岃€屽紓锛屾瘮濡傚湪绾跨矑浣撳唴鐨勯仐浼犲瘑鐮佷笌缁嗚優鏍告槸涓嶄竴鏍风殑銆?ldquo;Premier”鍙互閽堝妯℃澘DNA 鐨勬潵婧愪互鐩稿簲鐨勯仐浼犲瘑鐮佽鍒欒浆鎹NA 鍜屾皑鍩洪吀搴忓垪銆傝蒋浠跺叡缁欏嚭鍏鐢熺墿浜氱粨鏋勭殑涓嶅悓閬椾紶瀵嗙爜瑙勫垯渚涚敤鎴烽€夋嫨锛屾湁绾ゆ瘺铏ぇ鏍革紙Ciliate Macronuclear锛夈€佹棤鑴婃鍔ㄧ墿绾跨矑浣擄紙Invertebrate Mitochondrion锛夈€佹敮鍘熶綋锛圡ycoplasma锛夈€佹鐗╃嚎绮掍綋锛圥lant Mitoch- ondrion锛夈€佸師鐢熷姩鐗╃嚎绮掍綋锛圥rotozoan Mitochondrion锛夈€佷竴鑸爣鍑嗭紙Standard锛夈€佽剨妞庡姩鐗╃嚎绮掍綋锛圴ertebrate Mitochondrion锛夊拰閰垫瘝绾跨矑浣擄紙Yeast Mitochondrion锛夈€?br />2. 浣跨敤姝ラ鍙婃妧宸?br />“Premier”杞欢鍚姩鐣岄潰濡備笅锛?br /> 鍏朵富瑕佸姛鑳藉湪涓荤晫闈笂涓€鐩簡鐒讹紙鎸夐挳鍔熻兘濡備笂杩帮級銆傞檺鍒舵€ч叾鍒囩偣鍒嗘瀽鍙婂熀鍏冩煡鎵惧姛鑳芥瘮杈冪畝鍗曪紝鐐瑰嚮璇ュ姛鑳芥寜閽悗锛岄€夋嫨鐩稿簲鐨勯檺鍒舵€у唴鍒囬叾鎴栧熀鍏冿紙濡?10 搴忓垪锛?35 搴忓垪绛夛級锛屾寜纭畾鍗冲彲銆傚父瑙佺殑闄愬埗鎬у唴鍒囬叾鍜屽熀鍏冧竴鑸兘鍙互鎵惧埌銆備綘杩樺彲浠ョ紪杈戞垨鑰呮坊鍔犳柊闄愬埗鎬у唴鍒囬叾鎴栧熀鍏冦€?br />杩涜寮曠墿璁捐鏃讹紝鐐瑰嚮鎸夐挳锛岀晫闈㈠涓嬶細
        

          杩涗竴姝ョ偣鍑?img title="PCR?寮曠墿璁捐鍙奝remier?Primer?銆丱ligo?杞欢浣跨敤鎶€宸? height="28" alt="" src="http://www.bioask.cn/userfiles/image/primer3.gif" width="58" />鎸夐挳锛屽嚭鐜?ldquo;search criteria”绐楀彛锛屾湁澶氱鍙傛暟鍙互璋冩暣銆傛悳绱㈢洰鐨勶紙Seach For锛夋湁涓夌閫夐」锛孭CR 寮曠墿锛圥CR Primers锛夛紝娴嬪簭寮曠墿锛圫equencing Primers锛夛紝鏉備氦鎺㈤拡锛圚ybridization Probes锛夈€傛悳绱㈢被鍨?Search Type)鍙€夋嫨鍒嗗埆鎴栧悓鏃舵煡鎵句笂銆佷笅娓稿紩鐗╋紙Sense/Anti-sense Primer锛屾垨Both锛夛紝鎴栬€呮垚瀵规煡鎵撅紙Pairs锛夛紝鎴栬€呭垎鍒互閫傚悎涓娿€佷笅娓稿紩鐗╀负涓伙紙Compatible with Sense/Anti-sense Primer锛夈€傚彟澶栬繕鍙敼鍙橀€夋嫨鍖哄煙锛圫earch Ranges锛夛紝寮曠墿闀垮害锛圥rimer Length锛夛紝閫夋嫨鏂瑰紡锛圫earch Mode锛夛紝鍙傛暟閫夋嫨锛圫earch Parameters锛夌瓑绛夈€備娇鐢ㄨ€呭彲鏍规嵁鑷繁鐨勯渶瑕佽瀹氬悇椤瑰弬鏁般€傚鏋滄病鏈夌壒娈婅姹傦紝寤鸿浣跨敤榛樿璁剧疆銆傜劧鍚庢寜锛岄殢涔嬪嚭鐜扮殑Search Progress 绐楀彛涓樉绀篠earch Completed 鏃讹紝鍐嶆寜锛岃繖鏃舵悳绱㈢粨鏋滀互琛ㄦ牸鐨勫舰寮忓嚭鐜帮紝鏈変笁绉嶆樉绀烘柟寮忥紝涓婃父寮曠墿锛圫ense锛夛紝涓嬫父寮曠墿(Anti-sense)锛屾垚瀵规樉绀?Pairs)銆傞粯璁ゆ樉绀轰负鎴愬鏂瑰紡锛屽苟鎸変紭鍔ｆ搴忥紙Rating锛夋帓鍒楋紝婊″垎涓?00锛屽嵆鍚勬寚鏍囧熀鏈兘鑳借揪鏍囷紙濡備笅鍥撅級銆?br />       

       鐐瑰嚮鍏朵腑涓€瀵瑰紩鐗╋紝濡傜1#寮曠墿锛屽苟鎶婁笂杩扮獥鍙ｆ尓寮€鎴栭€€鍑猴紝鏄剧ず“Peimer Premier”涓荤獥鍙ｏ紝濡傚浘鎵€绀猴細
      

         璇ュ浘鍒嗕笁閮ㄥ垎锛屾渶涓婇潰鏄浘绀篜CR 妯℃澘鍙婁骇鐗╀綅缃紝涓棿鏄墍閫夌殑涓婁笅娓稿紩鐗╃殑涓€浜涙€ц川锛屾渶涓嬮潰鏄洓绉嶉噸瑕佹寚鏍囩殑鍒嗘瀽锛屽寘鎷彂澶圭粨鏋勶紙Hairpin锛夛紝浜岃仛浣擄紙Dimer锛夛紝閿欒寮曞彂鎯呭喌锛團alse Priming锛夛紝鍙婁笂涓嬫父寮曠墿涔嬮棿浜岃仛浣撳舰鎴愭儏鍐碉紙Cross Dimer锛夈€傚綋鎵€鍒嗘瀽鐨勫紩鐗╂湁杩欏洓绉嶇粨鏋勭殑褰㈡垚鍙兘鏃讹紝鎸夐挳鐢?img title="PCR?寮曠墿璁捐鍙奝remier?Primer?銆丱ligo?杞欢浣跨敤鎶€宸? height="24" alt="" src="http://www.bioask.cn/userfiles/image/primer8.gif" width="60" />鍙樻垚锛岀偣鍑昏鎸夐挳锛屽湪宸︿笅瑙掔殑绐楀彛涓氨浼氬嚭鐜拌缁撴瀯鐨勫舰鎴愭儏鍐点€備竴瀵圭悊鎯崇殑寮曠墿搴斿綋涓嶅瓨鍦ㄤ换浣曚竴绉嶄笂杩扮粨鏋勶紝鍥犳鏈€濂界殑鎯呭喌鏄渶涓嬮潰鐨勫垎鏋愭爮娌℃湁锛屽彧鏈?img title="PCR?寮曠墿璁捐鍙奝remier?Primer?銆丱ligo?杞欢浣跨敤鎶€宸? height="24" alt="" src="http://www.bioask.cn/userfiles/image/primer8.gif" width="60" />銆傚€煎緱娉ㄦ剰鐨勬槸涓棿涓€鏍忕殑鏈熬缁欏嚭璇ュ紩鐗╃殑鏈€浣抽€€鐏俯搴︼紝鍙弬鑰冨簲鐢ㄣ€?br />        鍦ㄩ渶瑕佸寮曠墿杩涜淇グ缂栬緫鏃讹紝濡傚湪5’绔姞鍏ラ叾鍒囦綅鐐癸紝鍙偣鍑?img title="PCR?寮曠墿璁捐鍙奝remier?Primer?銆丱ligo?杞欢浣跨敤鎶€宸? height="29" alt="" src="http://www.bioask.cn/userfiles/image/primer12.gif" width="57" />锛岀劧鍚庝慨鏀瑰紩鐗╁簭鍒椼€傝嫢瑕佸洖鍒版悳绱㈢粨鏋滀腑锛屽垯鐐瑰嚮鎸夐挳銆?/p>

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        鎬讳箣锛?ldquo;Premier”鏈変紭绉€鐨勫紩鐗╄嚜鍔ㄦ悳绱㈠姛鑳斤紝鍚屾椂鍙繘琛岄儴鍒嗘寚鏍囩殑鍒嗘瀽锛岃€屼笖瀹规槗浣跨敤锛屾槸涓€涓浉褰撲笉閿欑殑杞欢銆?br />Oligo 6.22 浣跨敤鎶€宸х畝浠?br />1. 鍔熻兘
         鍦ㄤ笓闂ㄧ殑寮曠墿璁捐杞欢涓紝“Oligo”鏄渶钁楀悕鐨勩€傚畠鐨勪娇鐢ㄥ苟涓嶅崄鍒嗗鏉傦紝浣嗗垵瀛﹁€呭鏄撹鍏跺鏉傜殑鍥捐〃鍚撳€掋€侽ligo 5.0 鐨勫垵濮嬬晫闈㈡槸涓や釜鍥撅細Tm 鍥惧拰ΔG 鍥撅紱Oligo 6.22 鐨勭晫闈㈡洿澶嶆潅锛屽嚭鐜颁笁涓浘锛屽姞浜嗕釜Frq 鍥俱€?ldquo;Oligo”鐨勫姛鑳芥瘮“Premier”杩樿鍗曚竴锛屽氨鏄紩鐗╄璁°€備絾瀹冪殑寮曠墿鍒嗘瀽鍔熻兘濡傛寮哄ぇ浠ヨ嚦浜庤兘椋庨潯鍏ㄤ笘鐣屻€?br />2. 浣跨敤锛堜互Oligo 6.22 涓轰緥锛?br />Oligo 6.22 鐨勫惎鍔ㄧ晫闈㈠涓嬶細
       

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