TheEpiQuik™ DNA Methyltransferase Activity/Inhibitor
Screening Assay Kitis a convenient set of tools that allows the
experimenter to selectively
screen Dnmt3B activity/inhibitors. The selective inhibition of
Dnmt3B may lead to demethylation and expression of the silenced
tumor suppressor genes. Thus the selective Dnmt3B inhibitors could
be new addition to cancer therapeutic agents. The kit is
ready-to-use and provides all the essential components needed to
carry out a successful Dnmt3B activity/inhibitor screening
experiment without the need for radioactivity or any special
equipment.
WHY CHOOSE THE EPIQUIK™ DNA METHYLTRANSFERASE 3B
ACTIVITY/INHIBITOR SCREENING ASSAY KIT?
Very rapid procedure, which can be completed
within 3 hours.
Safe and innovative colorimetric assay without
radioactivity, extraction, and chromatography.
Strip microplate format makes the assay flexible:
manual or high throughput analysis.
Extremely simple, reliable, and consistent assay
conditions.
Principle &
Procedure
The EpiQuik™ DNA Methyltransferase 3B Activity/Inhibitor Screening
Assay Kit is designed for screening Dnmt 3B inhibitors. In an assay
with this kit, the unique cytosine-rich DNA
substrate is stably coated on the strip wells. These wells are
specifically treated to have a high DNA absorption ability. Dnmt 3B
enzymes transfer methyl group to cytosine from Adomet
to
methylate DNA substrate. The methylated DNA can be recognized with
anti-5-methylcytosine antibody. The ratio or amount of methylated
DNA, which is proportional to enzyme activity, can then be
colorimetrically quantified through an ELISA-like
reaction.