咽部涂片发现革兰氏阴性双球菌不能诊断淋病0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">因为其他奈瑟菌属在咽部是正常的菌群0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">另外对症状不典型的涂片阳性应作进0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">步检查0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q931444.gif">）培养检查：

　　淋球菌培养是诊断的重要佐证0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">培养法对症状很轻或无症状的男性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q447608.gif">女性病人都是较敏感的方法0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">只要培养阳性就可确诊0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">在基因诊断问世以前0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">培养是世界卫生组织推荐的筛选淋病的唯0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">方法0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">目前国外推荐选择培养基有改良的Thayer-Martin（TM）培养基和New York City（NYC）培养基0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">国内采用巧克力琼脂或血琼脂培养基0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">均含有xxx0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">可选择地抑制许多其他xx生长0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">在0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">℃0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q929789.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">%湿度0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">含0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">%-0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">%CO0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">（烛缸）环境中培养0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">-0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q481387.gif">小时观察结果0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">培养后还需进行菌落形态0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">革兰氏染色0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">氧化酶试验和糖发酵试验等鉴定0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">培养阳性率男性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q481387.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">%-0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">%0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">女性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q481387.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">-0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">%0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q456817.gif">）抗原检测

　　0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">．固相酶免疫试验（EIA）：可用来检测临床标本中的淋球菌抗原0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">在流行率很高的地区而又不能作培养或标本需长时间远送时使用0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">可以在妇女人群中用来诊断淋球菌感染0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">．直接免疫荧光试验：通过检测淋球菌外膜蛋白I的单克隆抗体作直接免

　　疫荧光试验0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">但目前在男女0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q931444.gif">性标本的敏感不高0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">特异性差0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">加之实验人员的判断水平0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">故该实验尚不能推荐用来诊断淋球菌感染0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q313179.gif">）基因诊断

　　0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">．淋球菌的基因探针诊断

　　淋球菌的基因探针诊断0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">所用的探针有：质粒DNA探针0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">染色体基因探针和rRNA基因探针0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">）质粒DNA探针

　　① 隐蔽质粒DNA探针0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">淋球菌质粒分为0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q456817.gif">种：接合性质粒0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">分子{zd0}0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">为0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">kb DNA；  耐药性质粒包括两个质粒0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">DNA长分别为0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">kb和0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q929789.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">kb；隐蔽质粒0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">kb0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">其中隐蔽质粒存在于0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">%的临床淋球菌分离株中0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">其它奈瑟菌不含有此质粒0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">故可用它的序列作为特异DNA探针检测淋球菌0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">Torres采用核酸杂交技术检测淋球菌0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">所用探针为隐蔽质粒0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">用该探针对0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">株淋球菌和0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">株相关菌株的检测0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">有0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">株淋球菌杂交反应阳性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">占0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">%0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">还可与个别其它的奈瑟菌出现交叉反应0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">对测定探针的敏感性实验表明可检出0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">CFU淋球菌0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">研究证明隐蔽质粒中CPPB基因序列在所有的淋球菌染色体中（包括不含该质粒的菌株）0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">因此CPPB基因探针具有良好的特异性和敏感性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">Torres等用CPPB基因探针检测了0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">份临床标本0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif"> 采用非放射性地高辛标记系统0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif"> 其敏感性和特异性分别为0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">%和0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q481387.gif">%0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　②     耐药性质粒DNA探针

　　淋球菌的抗药质粒可分为：①产青毒素酶淋球菌（PPNG）其β-内酰胺酶阳性；②具有高水平的质粒介导的耐0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q313179.gif">环素淋球菌（TRNG）0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　PPNG菌株是0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q929789.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">年首次在实验室分离得到的0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">该菌中含有编码产青毒酶的基因0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">该基因既可整合于染色体上也可出现在质粒DNA中0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">而后者居多0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">称之为产青毒素酶质粒0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">质粒有0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q931444.gif">种0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">大小分别为0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q929789.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">kb和0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">kb0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">Pescador0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q481387.gif">年设计0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">特异的检测淋球菌编码β-内酰胺酶基因的探针0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">采用酶化学发光法标记0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">液相杂交0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">用测光计测是特异杂交体的光量0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">在0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">h内可检测0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">-0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">CFU的PPNG菌株0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">TRNG菌株虽对0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q313179.gif">环素耐药0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">但通常对β-内酰胺酶类及喹诺酮类xx素敏感0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">因此0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">在实验室药敏检测中可归为敏感xx0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">Pescador用抗0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q313179.gif">环素淋球菌(TRNG)tetm基因的寡核苷酸探针,该基因介导抗0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q313179.gif">环素,用酶化学发光标记,液相杂交,0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">h内可直接从临床标本中检出含有tetm基因的0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">×0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">CFu的淋球菌0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　(0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">)染色体探针

　　染色体探针包括已知功能的基因探针0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">如菌毛DNA探针和paI基因探针0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">这些基因在淋球菌感染人细胞的过程中起着重要作用；未知功能的基因探针0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">这些探针序列与染色体的特定序列互补0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">但目前还不知这些基因序列的功能0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">以上两种染色体探针由于在淋球菌中互补序列的拷贝数较低0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">检测灵敏度较低0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">因此0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">般用的不多0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">除非有特殊的研究目的0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">）rRNA基因探针

　　rRNA基因探针是将与rRNA互补的DNA作为探针0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">该探针的靶序列是rRNA序列0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">rRNA的基因探针的特点是：①可以增加探针检测的灵敏度0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">rRNA基因探针可同时检测rRNA分子和DNA分子；②rRNA具有进化上的保守性；③杂交方法简便0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q447608.gif">快速；④由于rRNA的含量较高0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">标本不需增菌0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">美国Gen-Probe公司生产的淋球菌检测探针PACE C0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">是以rRNA 及其基因为检测靶序列0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">采用放射性标记0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">在0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">h内可以完成检测0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">Peter用这种探针检测0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">个临床标本0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">结果灵敏度和特异性分别为0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">%0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">%0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">他认为PACE C系统筛查临床标本中淋球菌是0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">个可靠的方法0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">该探针还可以检测无症状淋球菌感染者0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">这是目前培养难于达到的0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q447608.gif">淋球菌的基因扩增检测

　　上面讲述的探针技术检测淋球菌的方法0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">虽然比培养方法在灵敏度0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">特异性和方便性上有了很大的提高0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">但其仍有0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">定的局限性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">如多数情况下需要标本的淋球菌浓度很高0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">PCR技术和连接酶链反应的出现进0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">步提高了检测淋球菌的灵敏性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">它具有快速0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q447608.gif">灵敏0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q447608.gif">特异0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q447608.gif">简便的优点0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">可以直接检测临床标本中极微量的病原体0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">）淋球菌DNA的提取

　　①培养菌的DNA提取

　　将培养得到的淋球菌0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">以0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">cfu/ml的浓度溶于碱性裂解液中裂解0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">裂解液组成为：0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">M NaCl 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">M NaOH和0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">%Sodium dodecyl sulphate0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">将裂解液混匀后煮沸0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">min0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">然后用0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">μl 的0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">M Tris pH0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q929789.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">中和碱性裂解液0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">用Tris平衡酚提抽0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">次0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">酚—氯仿抽提0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">次0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">然后用无水乙醇或异丙醇沉淀DNA0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">提取的DNA溶于0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">μl蒸馏水或TE缓冲液中0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　②临床棉拭子标本的提取

　　将贴有分泌物的棉拭子在0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">ml的无菌生理盐水中或PBS缓冲液中挤压洗0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">min0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">以便将标本尽量溶于溶液中0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">将棉拭子弃去0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">悬浮液于0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">-0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">r/min离心0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">min0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">吸去上清液0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">细胞重新溶于0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">μl 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">×PCR缓冲液0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">其中含Tween 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif"> 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">%,蛋白酶K 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">μg/ml0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">细胞悬浮液于0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">-0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">℃温浴0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">h,然后0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">℃加热0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">min以灭活蛋白酶K,0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif"> r/min离心0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">min,上清液含DNA模板0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">）PCR引物的设计

　　由于淋球菌隐蔽质粒CPPB基因在淋球菌染色体中和0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">kb隐蔽质粒中都有存在0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">同时在0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">%的淋球菌中都有该隐蔽质粒0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">因此很多PCR引物设计在CPPB基因区0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　靶基因                    引物序列                          片段长度（bp）

　　CPPB     NG0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">  0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">′GTT TGG CTG GTT GAT TCA AG 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">′                 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">

         NG0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">  0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">′GCA AGA TTT CCG ATTT GGC G 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">′

　　CPPB     HO0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif"> 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">′GCT ACG CAT ACC CGC GTT GC 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">′                  0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">

         HO0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif"> 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">′CGA AGA CCT TCG AGC AGA CA 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">′

　　rRNA    引物0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif"> 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">′-AGG CTG TTG CCA ATA TCG GC-0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">′                0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">

        引物0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">  0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">′-ACA CTC GAG TCA CCC AGT TC-0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">′

　　CPPB     GC0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif"> 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">′CTT ATC GTT TGG CTG GTT GAT TC 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">′              0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">

         GC0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif"> 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">′ACC AAG ACC AAA GGT TTG ACA CTG 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">′

         GC0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">  0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">′ATT TTC CAG TGT CAA AC 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">′                      0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">

         GC0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">  0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">′TAT TCA AGC CCT ATC TG 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">′

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">）PCR扩增

　　取淋球菌DNA提取液0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">μl0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">加入0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q481387.gif">μl的反应液中0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">最终PCR反应液中含dNTP各0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">μmol/L0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">引物各0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">μmol/L0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">TaqDNA聚合酶0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">U0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">Mg0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">+ 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">mmol/L,加无菌石蜡油0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">μl0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">r/min离心0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">s0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">进行PCR扩增循环0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">反应条件：0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">℃变性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">min0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">然后0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">℃ 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">s0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q929789.gif">℃0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">min0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q929789.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">℃0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">min0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">共0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">个循环0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">{zh1}0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q929789.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">℃延伸0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">min0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　扩增产物于0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">%的琼脂糖凝胶电泳0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">min0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">溴化乙锭染色0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">紫外灯下可见扩增的DNA荧光条带0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">分子大小应与所用引物扩增靶序列的大小0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">致0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">）PCR的灵敏性和特异性

　　由于CPPB在不含有隐蔽质粒的淋球菌染色体上也会含有CPPB基因0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">再加0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">%的淋球菌都会有隐蔽质粒0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">因此用CPPB作为靶序列的引物具有极高的敏灵性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">实验证明0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">般传统0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">步PCR（GC0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">-GC0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">）方法可以检出0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">个淋球菌0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">而用单管巢式PCR（GC0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">-GC0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">）可以检出≤0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">淋球菌（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">个CPPB基因）0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">这些引物经特异性实验0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">只能扩增淋球菌的DNA0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">而对非淋球菌奈瑟氏菌扩增不出特异产物0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">）单管巢式PCR方法

　　是在传统巢式PCR的基础上将两对PCR引物作特殊的设计0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">巢式外侧两个引物（GC0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">GC0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">）为0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">b退火温度比较高（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q481387.gif">℃）0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">巢式内侧两个引物GC0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">-GC0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">为0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q929789.gif">b退火温度较低（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">℃）0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">PCR反应液的其它成分与0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">般PCR相同0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">这样0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">通过控制退火温度（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q481387.gif">℃）使外侧引物先行扩增0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">经过0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">-0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">次循环后（第0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">次PCR）0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">再降低退火温度（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">℃）使内侧引物以第0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">次PCR产物为模板进行巢式扩增0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">该PCR的灵敏度可达到检出0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">个淋球菌0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">）淋球菌连接酶链反应（LCP）检测方法

　　目前PCR检测淋球菌的方法被广泛地使用0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">其特异性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">灵敏性不断提高0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">同时另0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">种基因诊断技术——连接酶链反应（LCP）也以其高特异0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">高灵敏性被应用于淋球菌的检测中0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">LCP 与PCR不同之处在于LCP 用0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q313179.gif">对引物0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">所用酶是连接酶0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">连接酶可以将两条相邻引物连接起来0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">连接起来的两条引物可以作为另两条引物的模板0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">后者在连接酶作用下连接0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">又可作为模板0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">如此进行0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">-0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">次循环0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">LCP所用的模板处理方法与PCR模板制备相等0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">LCP所用的探针除了可以设计在CPPB基因上0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">也可以设计在染色体基因序列上0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">例如opa-0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">基因0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">美国Abbott实验室在opa-0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">基因的0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q481387.gif">bp长的区域内设计了0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">个LCP探针0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">由于opa-0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">基因在淋球菌的染色体中有0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">次重复0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">因此0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">该组LCP探针具有高灵敏性和特异性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">LCP反应过程：

　　将模板加入LCP反应液中0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">LCP反应液：0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">mmol/L  Tris-HCl pH0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q929789.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">;0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif"> mmol/L KCl 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif"> mmol/L MgCl0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">；0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif"> mmol/L EDTA; 0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif"> mmol/L NAD+;0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif"> mmol/L DTT,有标记物的两个相邻探针各0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">fmol/L0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">未标记的探针各0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">fmol/L0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">U耐热连接酶0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">反应条件：0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q929789.gif">℃0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">s0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">℃0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">s0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q174348.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">℃0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">s0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">其0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">循环0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">μl反应产物加入酶标板微孔中0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">进行显色反应0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">{zh1}用酶标仪读取光值0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">根据Buimer的实验证明0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">LCP在检测男性尿道棉拭子标本的灵敏度为0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">%0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">尿液标本为0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q481387.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q481387.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">%0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">女性宫颈棉拭子标本为0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q304152.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q358143.gif">.0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q177731.gif">%0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">LCP方法的特异性高达0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q558433.gif">%0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">这0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">点明显高于PCR的特异性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">避免了假阳性的发生0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">． 临床基因诊断淋球菌的注意事项

　　目前临床检测淋球菌的基因诊断方法主要采用PCR方法0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">但是该方法在临床检测中应注意几个问题0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q683909.gif">）引物设计 除了以上所列的淋球菌PCR引物外0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">还可从在其它基因上设计0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">但

　　是引物序列应具有特异性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">因为xx的染色体较大0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">许多基因序列并没有搞清楚；同时xx之间或近或远有0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">定的同源性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">而且xx所含质粒序列间也存在同源性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">因此设计引物0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">定要进行基因数据库比较分析0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">同时进行特异性和灵敏性实验0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">从中选择引物进行临床检测0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q538760.gif">）临床标本处理 对临床标本来讲0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">PCR模板要求越纯越好0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">这就要求在采集标本时要取到准确的位置0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">对于无症状患者要适当多采样0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">以保证采集到病原体xx0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">另外由于临床标本成分比较复杂0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">有时简单处理的标本PCR扩增效果并不理想0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">这可能是由于杂质过多造成的,需要进0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">步纯化0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">如用酚0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">氯仿抽提法纯化0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">结果将会好转0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">这种纯化方法比较麻烦,目前已有商品化的DNA纯化试剂盒,可以较简便地从临床标本中提取高纯度的DNA0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q978023.gif">）PCR产物的检测方法  不久以前临床PCR检测淋球菌几乎都采用电泳的方法进行PCR产物的鉴定0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">该方法存在许多问题0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">如由于肉眼观察的主观性而xxx阳性和假阴性的结果0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">目前用杂交显色法代替电泳法0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">提高了结果判断的特异性和灵敏性0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　总之0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">PCR方法与LCP方法比传统的培养法在灵敏性和特异性上有了很大的提高0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">时间也大大缩短0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">随着基因诊断技术的不断改进0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">PCR方法与LCP方法在淋球菌的检测将会成为常规的检测方法0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q198628.gif">）药敏试验：在培养阳性后进0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q700813.gif">步作药敏试验0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">用纸片扩散法做敏感试验0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">或用琼脂平皿稀释法测定最小抑菌浓度（MIC）0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">用以指导选用xxx0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q761301.gif">

　　（0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q790679.gif">）PPNG检测：β-内酰胺酶0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">用纸片酸度定量法0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">使用Whatman I号滤纸PP-NG

　　菌株能使其颜色由蓝变黄0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">阳性为PPNG0 && image.height>0){if(image.width>=700){this.width=700;this.height=image.height*700/image.width;}}" src="http://jb.qm120.com/jb/0711120912559917/q258648.gif">阴性为N-PPNG