【产品详细说明】

Product Overview

The EpiQuik™ Global Histone H3-K9 Methylation Assay Kit is suitable for specifically measuring global histone H3-K9 methylation using a variety of mammalian cells includingfresh and frozen tissues, cultured adherent and suspension cells.

WHY CHOOSE THE EPIQUIK™ GLOBAL HISTONE H3-K9 METHYLATION ASSAY KIT?

• Quick and efficient, which can be finished within 5 hours.
• Innovative colorimetric assay with no need for radioactivity, electrophoresis, and chromatography.
• Strip microwell format makes the assay flexible: manual or high throughput.
• Simple, reliable, and consistent assay conditions.

Principle & Procedure

The EpiQuik™ Global H3-K9 Methylation Assay Kit is designed for measuring global histone H3-K9 methylation. In an assay with this kit, the histone proteins are stably spotted on the strip wells. The methylated histone H3-K9 can be recognized with high-affinity antibody. The ratio or amount of methylated H3-K9 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color    development.

Product Components

GC1 (10x lysis buffer)
GC2 (Extraction buffer)
GC3 (10x wash buffer)
GC4 (Histone buffer)
GC5 (Blocking buffer)
GC6 (Antibody buffer)
GC7 (capture antibody, 100 µg/ml)*
GC8 (detection antibody, 400 µg/ml)*
GC9 (developing solution)
GC10 (stop solution)
Methylated H3-K9 control (60 µg/ml)*
8 well assay strips (with frame)
User guide

* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.

User Guide & MSDS

References & Citations

Hu, Nan et al. (May2008). Abnormal histone modification patterns in lupus CD4+ T cells. J Rheumatol. 35(5): 804-10.