ELISA鎿嶄綔甯歌闂

ELISA鏂规硶琚箍娉涘簲鐢ㄤ簬鍚勭鎶楀師鍜屾姉浣撴祴瀹氥€備絾ELISA娴嬪畾涓奖鍝嶅洜绱犺緝澶氾紝鑰屼笖鍏舵搷浣滀腑鏈変竴瀹氱殑鎶€鏈姹傦紝鍦ㄦ娴嬭繃绋嬩腑闄ゆ甯稿弽搴斿锛屾湁鏃跺父瑙佸埌涓€浜涢敊璇殑缁撴灉銆傚紩璧?/span>ELISA娴嬪畾閿欒缁撴灉鐨勫師鍥犱富瑕佹湁锛?/span>鈶?/span>鏍囨湰鍥犵礌锛?/span>鈶?/span>璇曞墏鍥犵礌锛?/span>鈶?/span>鎿嶄綔鍥犵礌銆備笅闈㈠氨涓€浜涘父瑙?/span>ELISA鎿嶄綔杩囩▼涓殑闂涓€涓€鍒嗘瀽銆?/span>
1锛庢牱鍝佺█閲?/span>
閰惰仈鍏嶇柅鍙嶅簲鏄竴绉嶆晱鎰熸€у緢楂樼殑鍙嶅簲锛屽鏋滆娓呬笉绋€閲婏紝涓嶅彲閬垮厤浼氫骇鐢熷緢寮虹殑闈炵壒寮傛€у弽搴旓紝鍑虹幇鍋囬槼鎬с€傚洜姝わ紝鍥藉妫€娴嬭娓呮姉浣撶殑璇曞墏鐩掞紝鍧囪瀹氬皢琛€娓呯█閲婅嚦閫傚綋鐨勫€嶆暟锛屼互闄嶄綆闈炵壒寮傛€у弽搴旓紝浣跨壒寮傛€х殑鎶楀師鎶椾綋鍙嶅簲鍏呭垎浣撶幇鍑烘潵銆備竴鑸骇鍝佺殑鏍峰搧绋€閲婂€嶆暟鍧囬€氳繃澶ч噺璇曢獙纭畾锛屼互淇濊瘉璇曢獙鐨勭伒鏁忓害鍜岀壒寮傛€с€備絾鏄紝鏈変簺鐢ㄦ埛鏈兘涓ユ牸鎸変娇鐢ㄨ鏄庝功鎿嶄綔锛屽鏌愪簺浜у搧搴斿彇10μl鏍峰搧杩涜绋€閲婏紝涓埆鐢ㄦ埛鍙?/span>5μl鐢氳嚦1μl鏍峰搧杩涜绋€閲婏紝鐢变簬鍚稿槾涓婁笉鍙伩鍏嶅湴娌炬湁鏍峰搧浠ュ強寰噺绉绘恫鍣ㄧ殑绮惧害涓嶅锛屽洜姝ら€犳垚鏍峰搧绋€閲婂€嶆暟涓嶅噯纭紝妫€娴嬬粨鏋滃嚭鐜伴棶棰樸€?/span>
2锛庤瘯鍓傜洅骞宠　
璇曞墏鐩掍腑鎵€鏈夎瘯鍓傚拰鏉挎潯鍧囧簲鍦ㄨ瘯楠屽墠骞宠　鑷冲娓?/span>(绾?/span>25鈩?/span>)锛屼竴鑸渶鍦ㄥ娓╂斁缃?/span>20锝?/span>30鍒嗛挓浠ヤ笂銆傚钩琛℃椂闂村お鐭細閫犳垚璇曞墏娣峰寑涓嶅锛屾牱鍝佸鑲叉椂闂寸浉瀵圭缉鐭紝ELISA鍙嶅簲涓嶅鍏呭垎銆傚啲瀛ｅ娓╀綆锛屽彲灏嗚瘯鍓傜洅缃?/span>37鈩?/span>娓╃20鍒嗛挓銆?/span>
3锛庢牱鍝佸拰璇曞墏鐨勬贩鍖€
绋€閲婂墠銆佸悗鐨勬牱鍝佸繀椤诲厖鍒嗘贩鍖€锛屾墍鏈夎瘯鍓傚湪鍔犳牱鍓嶄篃椤绘憞鍖€锛屼互淇濊瘉璇曢獙鐨勫潎涓€鎬с€?/span>
4锛庡姞鏍?/span>
鍦ㄧ幇鍦ㄧ殑ELISA鍟嗗搧璇曞墏鐩掍腑锛屽繀鐒舵湁浣跨敤寰噺鍔犳牱鍣ㄥ姞鍏ユ牱鏈殑姝ラ銆傛敞鎰忕殑鍏抽敭鐐规槸锛氬姞鏍蜂笉鍙お蹇紝瑕侀伩鍏嶅姞鍦ㄥ瓟澹佷笂閮紝涓嶅彲婧呭嚭鍜屼骇鐢熸皵娉°€傚姞鏍峰お蹇紝鏃犳硶淇濊瘉寰噺鍔犳牱鐨勫噯纭€у拰鍧囦竴鎬с€傚姞鍦ㄥ瓟澹佷笂閮ㄧ殑闈炲寘琚尯锛屾槗瀵艰嚧闈炵壒寮傚惛闄勩€傛簠鍑轰細瀵归偦杩戝瓟浜х敓姹℃煋銆傚嚭鐜版皵娉″垯鍙嶅簲娑茬晫闈㈡湁宸紓銆傛墍浠ワ紝鏈夋椂鍊欎竴浠芥爣鏈敤鐩稿悓鐨勮瘯鍓傜洅杩欐娴嬪畾涓洪槼鎬э紝涓嬫娴嬪畾涓洪槾鎬э紝寰€寰€灏辨槸涓婅堪鍔犳牱鍙婅瘯鍓傜殑閿欒鎵€鑷淬€?/span>
5锛庢俯鑲?/span>
娓╄偛鏄?/span>ELISA娴嬪畾涓奖鍝嶆祴瀹氭垚璐ユ渶涓哄叧閿殑涓€涓洜绱犮€?/span>ELISA浣滀负涓€绉嶅浐鐩稿厤鐤祴瀹氾紝鎶楀師鎶椾綋鐨勭粨鍚堝弽搴斿湪鍥虹浉涓婅繘琛岋紝瑕佷娇娑茬浉涓殑鎶楀師鎴栨姉浣撲笌鍥虹浉涓婄殑鐗瑰紓鎶椾綋鎴栨姉鍘熷畬鍏ㄧ粨鍚堬紝蹇呴』鍦ㄤ竴瀹氱殑娓╁害鏉′欢涓嬪弽搴斾竴瀹氱殑鏃堕棿銆傛俯鑲叉墍闇€鏃堕棿涓庢俯搴︽垚鍙嶆瘮锛屽嵆娓╁害瓒婇珮锛屽垯鎵€闇€鏃堕棿鐩稿杈冪煭銆傛渶涓哄父鐢ㄧ殑娓╄偛娓╁害鏈?/span>37鈩?/span>鍜屽娓╋紝鍏舵鏄?/span>43鈩?/span>鍜?/span>2锝?/span>8鈩?/span>銆備竴浜涙搷浣滆€咃紝鎿呰嚜鏀瑰彉璇存槑涔︽搷浣滐紝浣跨敤鑷繁鍠滄鐨勬俯鑲叉椂闂村拰娓╄偛娓╁害锛岃繖鏍烽€犳垚涓€浜涗笉蹇呰鐨勯夯鐑︺€傚洜涓猴紝涓嶅悓璇曞墏鐩掓湁涓嶅悓鐨勬俯鑲叉椂闂村拰娓╄偛娓╁害鐨勯€夋嫨锛岄殢鎰忔洿鏀规俯鑲叉椂闂存垨鑰呮俯鑲叉俯搴﹀皢瀵艰嚧璇曢獙缁撴灉鍑虹幇鍋忓樊銆?/span>
6锛庢礂鏉?/span>
鍥虹浉鍏嶇柅娴嬪畾鎶€鏈槸涓€绉嶉潪鍧囩浉鍏嶇柅娴嬪畾鎶€鏈紝闇€浠ユ礂娑ゆ搷浣滃皢鐗瑰紓缁撳悎浜庡浐鐩哥殑鎶楀師鎴栨姉浣撲笌鍙嶅簲娓╄偛杩囩▼涓惛闄勭殑闈炵壒寮傛垚浠藉垎绂诲紑鏉ワ紝浠ヤ繚璇?/span>ELISA娴嬪畾鐨勭壒寮傛€с€傛礂鏉垮浜?/span>ELISA娴嬪畾鏉ヨ锛屼篃鏄瀬鍏跺叧閿殑涓€姝ャ€傛礂娑插敖閲忎笉瑕佹孩鍑哄瓟澶栵紱鍔犳礂娑插悗瑕侀潤缃?/span>1鍒嗛挓锛岀敥鍘绘澘瀛斾腑娲楁恫鍚庯紝涓€瀹氳澶у姏鎷嶅共锛涘強鏃舵洿鎹㈠惛姘寸焊锛屽挨鍏舵槸鎷嶈繃閰舵爣璁扮墿鐨勫惛姘寸焊涓€瀹氳寮冨幓锛屽惁鍒欏彲鑳藉奖鍝嶈瘯楠岀粨鏋溿€?/span>
7锛庤竟缂樻晥搴?/span>
浣跨敤96瀛旀澘鐨?/span>ELISA娴嬪畾涓紝甯稿彂鐜版湁“杈圭紭鏁堝簲”锛屽嵆澶栧懆瀛旀樉鑹茶緝涓績瀛旀繁銆傜粡鐮旂┒璇佸疄鍦ㄦ俯鑲蹭腑鐨勭儹鍔涘姊害鍙兘鏄牴鏈師鍥犱箣鎵€銆傝仛鑻箼鐑湰韬负涓嶈壇鐑浣擄紝鍦ㄥ疄楠屽鐨勫父瑙?/span>ELISA娴嬪畾涓紝灏嗘澘浠庡娓╋紙閫氬父鍦?/span>25鈩?/span>宸﹀彸锛夌疆浜?/span>37鈩?/span>娓╃锛屾澘涔熷崌娓╂椂锛屽湪澶栧懆瀛斾笌涓績瀛斾箣闂村彲鑳藉瓨鍦ㄤ竴鐑姏瀛︽搴︺€傚洜姝や娇鐢ㄦ按娴存垨鍦ㄥ皢鍙嶅簲婧舵恫鍔犲叆鑷虫澘瀛斾腑鏃讹紝灏嗘澘鍜屾憾娑插潎鍔犵儹鑷虫俯鑲叉俯搴︼紙濡?/span>37鈩?/span>锛夛紝灏卞彲浠ュ緢瀹规槗鍦版帓闄?/span>“杈圭紭鏁堝簲”锛屽苟涓斿彲鎻愰珮娴嬪畾鐨勯噸澶嶆€с€?/span>
8锛庢樉鑹?/span>
鏄捐壊涓€瀹氳鎺у埗鏃堕棿,鏍规嵁璇曞墏鐩掕鏄庢搷浣滃嵆鍙€備竴鑸潵璇达紝鏄捐壊鏃堕棿杩囩煭锛岀粨鏋滃亸浣庯紱鏄捐壊鏃堕棿杩囬暱锛岀┖鐧藉楂樻垨鑰呴潪鐗瑰紓鎬ф樉鑹插鍔犮€?/span>
9锛庢瘮鑹?/span>
姣旇壊瑕佹敞鎰忔尝闀跨殑閫夋嫨銆備互TMB涓哄簳鐗╁拰浠?/span>OPD涓哄簳鐗╃殑璇曞墏鐩掑潎鏈変娇鐢紝鑰屽墠鑰呮瘮鑹叉尝闀夸负450nm锛屽悗鑰呬负492nm锛屾护鍏夌墖闇€鏍规嵁瑕佹眰闅忔椂鏇存崲銆傚洜姝わ紝瀹规槗鍑虹幇婊ゅ厜鐗囬敊鐢ㄧ殑闂銆?/span>
鍏舵锛屽崟娉㈤暱鎴栧弻娉㈤暱姣旇壊閫夋嫨鐨勯棶棰樸€傛墍璋撶殑鍗曟尝闀挎瘮鑹插嵆鏄€氬父鐨勪互瀵规樉鑹插叿鏈夋渶澶у惛鏀剁殑娉㈤暱濡?/span>450nm鎴?/span>492nm杩涜姣旇壊娴嬪畾锛涜€屽弻娉㈤暱鍙岃壊鍒欓叾鏍囦华鍦ㄦ晱鎰熸尝闀垮450nm鍜岄潪鏁忔劅娉㈤暱濡?/span>630nm涓嬪悇娴嬪畾涓€娆★紝鏁忔劅娉笂涓嬬殑鍚稿厜搴︽祴瀹氬€间负鏍锋湰娴嬪畾閰跺弽搴旂壒寮傛樉鑹茬殑鍚稿厜搴︿笌鏉垮瓟涓婃寚绾广€佸埉鐥曘€佺伆灏樼瓑鑴忕墿鎵€鑷寸殑鍚稿厜搴︿箣鍜岋紱闈炴晱鎰熸尝闀夸笅娴嬪畾鍗虫敼鍙樻尝闀胯嚦涓€瀹氬€硷紝浣垮緱鏍锋湰娴嬪畾閰跺弽搴旂壒寮傛樉鑹茬殑鍚稿厜搴﹀€间负闆讹紝姝ゆ椂娴嬪緱鐨勫惛鍏夊害鍗充负鑴忕墿鐨勫惛鍏夊害鍊笺€傛渶鍚庨叾鏍囦华缁欏嚭鐨勬暟鍊间负鏁忔劅娉㈤暱涓嬬殑鍚稿厜搴﹀€间笌闈炲父鎰熸尝闀夸笅鐨勫惛鍏夊害鍊肩殑宸€傚洜姝わ紝鍙屾尝闀挎瘮鑹叉祴瀹氬叿鏈夎兘鎺掗櫎鐢卞井婊存澘鏈韩銆佹澘瀛斿唴鏍囨湰鐨勯潪鐗瑰紓鍚告敹銆佹寚绾广€佸埉鐥曘€佺伆灏樼瓑瀵圭壒寮傛樉鑹叉祴瀹氬惛鍏夊害鐨勫奖鍝嶇殑浼樼偣銆傜敱浜?/span>ELISA娴嬪畾涓崟涓┖鐧藉瓟鐨勯潪鐗瑰紓鍚告敹涓婃湁涓€瀹氱▼搴︾殑涓嶇‘瀹氭€э紝涔熷氨鏄姣忔娴嬪畾鎴栧悓娆℃祴瀹氱┖鐧藉瓟浣嶇疆鐨勪笉鍚屽潎鏈夊彲鑳藉緱鍒颁笉鍚屽惛鍏夊害娴嬪畾鍊硷紝鏁呰€屽湪ELISA娴嬪畾姣旇壊鏃讹紝鏈€濂芥槸浣跨敤鍙屾尝闀挎瘮鑹层€?/span>

缁间笂鎵€杩帮紝灏界ELISA娴嬪畾鐨勬搷浣滄楠ら潪甯哥畝鍗曪紝浣嗘湁鍙兘浼氬奖鍝嶆祴瀹氱粨鏋滅殑鍥犵礌鍗磋緝澶氾紝鍒嗗竷鍦ㄦ祴瀹氭搷浣滅殑鍚勬涔嬩腑锛屽挨浠ュ姞鏍枫€佹俯鑲插拰娲楁澘涓虹敋銆備负甯姪澶у鍒嗘瀽鏌ユ壘娴嬪畾涓嚭鐜伴棶棰樼殑鍙兘鍘熷洜锛岀壒瀵瑰父瑙侀棶棰樺強鍘熷洜褰掔撼鎬荤粨浜庝笅琛ㄣ€?/span>

鎿嶄綔杩囩▼涓彲鑳藉嚭鐜扮殑闂鍜岃В鍐虫柟娉?/span>