【产品详细说明】

Product Overview

The EpiQuik™ General Protein-DNA Binding AssayKit (Fluorometric) (96 assays) is a convenient package of tools that allows the experimenter to investigate protein-DNA interaction in vitro efficiently. The entire procedure can be completed within 3 hours and produces far superior results than any competitor kits. The EpiQuik™ General Protein-DNA Binding Assay Kits are suitable for analyzing protein-DNA interactions in vitro, specifically for detecting transcription factor activation using mammalian tissue and cell extracts.

WHY CHOOSE THE EPIQUIK™ GENERAL PROTEIN-DNA BINDING ASSAY KIT (FLUOROMETRIC)(96 ASSAYS)?

• The fastest procedure available: a complete assay can be finished within 3 hours.
• Strip microplate  format makes the assay flexible: manual or high throughput analysis.
• Fluorometrically qualify or quantify protein activation and use radioactive-free materials: safer to handle.
• No requirement for coating antibodies, or for using secondary antibodies, or for labeling oligo-nucleotides: save time and reduce labor.
• Simple, reliable, and consistent assay conditions.

Principle & Procedure

The EpiQuik™ General Protein-DNA Binding Assay Kit (Fluorometric) is designed for measuring the transcription factor (TF) of DNA binding activity in nuclear extracts. In an assay with the use of this kit, target TF in nuclear extract is captured by high affinity antibody onto assay microwell. A double strand oligonucleotide (oligo) containing DNA binding consensus sequence for the target TF is added into the microwell and bound to active TF in the binding assay buffer. The bound oligo is then fluorometrically detected.

Product Components

PF1 (10x wash   buffer)
PF2 (antibody buffer)
PF3 (extract cleaner)*
PF4 (assay buffer)
PF5 (Oligo release solution)
PF6 (fluoro developer)*
DTT (500x)*
96-well assay plate
8-well assay strips (with frame)
User guide

User Guide & MSDS