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"Figure 1. A: Comparison of DNA staining by PI, TOTO-3, TOPRO-3, Hoechst 33258, and DRAQ5 with H2B-GFP chromatin labeling in HeLa cells. For all dyes the H2B-GFP chromatin label and DNA staining were imaged in interphase and mitotic cells. The three fixed-cell dyes TOTO-3, TOPRO-3, and PI were imaged with or without RNAse digestion. Dye concentrations are as listed in Table 1. For Hoechst and DRAQ5, only live cell images are shown. In all cases, confocal optical sections are presented with the corresponding phase contrast images. B: Combined imaging of DRAQ5 and two different autofluorescent proteins. Living C2C12 mouse myoblast cells expressing the cell cycle marker GFP-PCNA and a protein (MeCP2-mRFP) that binds to pericentric heterochromatin were stained with DRAQ5. The latter also shows that DRAQ5, in contrast to Hoechst/DAPI dyes, does not preferentially stain this type of heterochromatin. The three different fluorophores were excited and detected independently (imaging conditions as listed in Table 2 and no cross-talk was observed. Scale bars = 5." (寮曡嚜鍘熸枃/quoted from original paper)

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