【产品详细说明】

Product Overview

The EpiQuik™ MBD2 Binding Activity/Inhibition Assay Kit is a convenient set of tools that allows the experimenter to measure binding activity of MBD2 to methylated DNA. The kit is ready-to-use and provides all the essential components needed to carry out a successful activity/inhibition experiment without the need for radioactivity or any special equipment. The EpiQuik™ MBD2 Binding Activity Assay Kit is suitable for measuring MBD2 binding activity in human cells/tissues.

WHY CHOOSE THE EPIQUIK™ MBD2 BINDING ACTIVITY/INHIBITION ASSAY KIT?

• The fastest procedure available, which can be finished within 3 hours.
• Innovative colorimetric assay to quantitatively measure MBD2 binding.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Simple, reliable, and consistent assay  conditions.

Principle & Procedure

The EpiQuik™ MBD2 Binding Activity Assay Kit is designed for measuring in vitro or intracellular MBD2 binding activity. In an assay with this kit, Activated MBD2 protein contained in nuclear extract binds to methylated DNA and forms the MBD2/methylated DNA complex. The complex is then captured to specific-treated strip well plate.   MBD2 is recognized with the specific antibody and measured colorimetrically.

Product Components

MB1 (10x wash buffer)
MB2 (assay buffer)
MB3 (methylated DNA 25 µg/ml)*
MB4 (affinity antibody 50 µg/ml)*
MB5 (detection antibody 200 µg/ml)*
MB6 (developing solution)
MB7 (stop solution)
8 well assay strips (with frame)
User guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS