| 1.缁勭粐宸ョ▼瀵圭敓鐗╁弽搴斿櫒鐨勮姹?
鍔ㄧ墿缁嗚優灏哄鍦?0~30mm涔嬮棿锛屾瘮澧炴畺閫熺巼杈冩參(绾?.06hr-1)锛屽€嶅鏃堕棿闀?绾?2hr)銆備互鐢熺墿鍙嶅簲鍣ㄥ煿鍏荤殑缁嗚優瀵嗗害涓?06涓?mL(绾?.3g/L)锛岃緝楂樼粏鑳炲瘑搴︿负108涓?mL(绾?0g/L)銆傜粏鑳炵殑鑰楁哀閲忚緝澶绾?.3mmol/(L×hr)]锛屽叾鏈€澶у€煎彲杈惧埌30mmol/(L×hr)銆傜粍缁囧伐绋嬬敓鐗╁弽搴斿櫒鐨勫煿鍏诲弬鏁帮紝闄や簡閰嶅悎鍓嶈堪缁嗚優鐨勭壒鎬у強闇€姹傚锛岄』婊¤冻浠ヤ笅鐨勫熀鏈姹傦細鐢熺墿鍙嶅簲鍣ㄧ殑璁捐搴旀柟渚夸簬鍩瑰吇娑茬殑鍧囧寑娣峰悎锛屽苟鎻愪緵绮剧‘鐨勬帶鍒讹紝浣垮悇钀ュ吇鎴愬垎鍜屽煿鍏绘恫鐨刾H鍊兼搴﹀敖閲忓噺灏戯紝姝ゅ姩鎬佸煿鍏荤殑鏂瑰紡涔冧负浜嗕娇鏀灦(鍗崇粍缁囧潡)鍐呯殑姘у強钀ュ吇璐ㄤ紶鏇村厖鍒嗭紝浠ｈ阿浜х墿鏇村鏄撴帓鍑猴紝缁嗚優琛ㄥ瀷鏇村厖鍒嗗湴琛ㄨ揪銆傚煿鍏绘恫娣峰悎鏂瑰紡搴斾娇鍓簲鍔涘缁嗚優鐨勬崯浼ら檷鍒版渶浣庛€傝嫢涓哄共缁嗚優鍒嗗寲涔嬪ぇ閲忓煿鍏伙紝蹇呴』娉ㄦ剰绮惧噯鎺у埗骞茬粏鑳炲湪涓嶅悓鐨勫垎鍖栭樁娈垫墍闇€涓嶅悓鐨勭敓闀垮洜瀛愭祿搴︺€傜敓鐗╁弽搴斿櫒璁捐瑕佽兘鎺ヨ繎浣撳唴鍙戣偛鎯呭喌锛岃€屼綋鍐呭悇绉嶇粍缁囧拰鍣ㄥ畼鎵€澶勭幆澧冨悇寮傦紝鏌愪簺绉嶇被缁嗚優瑕佹眰鍙嶅簲鍣ㄨ兘鎻愪緵涓€瀹氱殑鏈烘搴斿姏锛屽鑲岃叡鍦ㄤ綋鍐呬富瑕佹壙鍙楀紶搴斿姏锛岃€岄楠间富瑕佹壙鎷呭帇搴斿姏锛屽績琛€绠′富瑕佹壙鍙楄剦鍔ㄥ紡搴斿姏锛屽叾瀹冨彲鑳界殑鐗╃悊褰卞搷鍥犲瓙灏氭湁纾佺晠顙辩數鍦虹瓑銆傚洜姝わ紝鐢熺墿鍙嶅簲鍣ㄧ殑璁捐蹇呴』鑳藉洜搴斾笉鍚岀粍缁囷紝鎻愪緵銆屼笓涓€鎬с€嶇殑闇€姹傘€傛澶栵紝涓烘弧瓒宠緝澶т綋绉粍缁囧缓鏋勫绉嶅瓙缁嗚優鏁伴噺鐨勯渶姹傦紝甯稿皢鐢熺墿鍙嶅簲鍣ㄤ笌寰浇浣撶粏鑳炲煿鍏绘妧鏈粨鍚堛€傜敱浜庡井杞戒綋鏈夎緝澶х殑姣旇〃闈㈢Н锛岀浉鍚屽绉殑鐢熺墿鍙嶅簲鍣ㄦ瘮鏅€氬煿鍏绘柟寮忥紝澶氬嚭鏁板崄鍊嶇敋鑷充笂鐧惧€嶇殑缁嗚優銆傚井杞戒綋鍩瑰吇缁嗚優鍙噺灏戠粏鑳炲幓鍒嗗寲鐜拌薄锛屾湁鍒╀簬缁嗚優琛ㄥ瀷鐨勭淮鎸侊紝鎻愰珮绉嶅瓙缁嗚優鐨勮川閲忋€傚洜姝わ紝鐢熺墿鍙嶅簲鍣ㄨ繕闇€瑕佷繚鎸佺瀛愮粏鑳炰笌寰浇浣撳湪鍩瑰吇杩囩▼涓壇濂界殑缁撳悎銆傚敖绠″缁勭粐宸ョ▼鍙嶅簲鍣ㄦ湁涓婅堪鑻涘埢鐨勮姹傦紝鐜伴樁娈典娇鐢ㄧ殑鍙嶅簲鍣紝澶ч儴鍒嗘槸鍦ㄦ棦鏈夊姩鐗╃粏鑳炲弽搴斿櫒涓婁綔灏戦儴鍒嗙殑鏀硅繘锛岃櫧鐒跺熀鏈笂鑳界鍚堝煿鍏婚渶姹傦紝浣嗙敱浜庝笓涓€鎬т笉寮猴紝鐢氳嚦瀛樺湪寰堝闂锛屽洜姝ょ敓鐗╁弽搴斿櫒鍦ㄧ粍缁囧伐绋嬮鍩熻繕鏈夊緢澶х殑鍒涙柊鍜屽彂灞曠┖闂淬€?
2.缁勭粐宸ョ▼鐨勭敓鐗╁弽搴斿櫒绉嶇被
2.1鎼呮媽寮忕敓鐗╁弽搴斿櫒
鎼呮媽寮忕敓鐗╁弽搴斿櫒(spinner flask bioreactors) 搴旂敤浜庡井杞戒綋缁嗚優鍩瑰吇锛屼互鍙婅蒋楠ㄥ拰鐨偆绛夌粍缁囧煿鍏汇€傚叾涓昏鍘熺悊鏄€氳繃妗ㄥ紡鎼呮媽鍣ㄦ潵鎼呭姩鍩瑰吇娑诧紝浠ュ鍔犺川浼犳晥鏋滐紝纭繚鍩瑰吇娑茬殑鍏诲垎鍜屾憾姘ф祿搴︾殑鍧囧寑鍒嗗竷锛岃揪鍒板ぇ瑙勬ā鍩瑰吇缁嗚優鐨勭洰鐨勩€傚拰鍏跺畠鐢熺墿鍙嶅簲鍣ㄧ浉杈冿紝鍏剁粨鏋勭畝鍗曘€佹垚鏈緝浣庛€傜粍缁囧潡鍩瑰吇鏃讹紝鎶婂凡瀹屾垚绉嶆缁嗚優鐨勬敮鏋舵偓鍚婂湪鐡跺涓婏紝浣垮叾娴告病鍦ㄥ煿鍏绘恫涓煿鍏汇€傚湪鎺ョ闃舵锛岀粏鑳炶棄鐢辨祦浣撳娴侊紝灏嗙粏鑳炶繍閫佽嚦鏀灦璐撮檮銆傚湪鍩瑰吇杩囩▼涓紝鍩瑰吇娑插洜鎼呮媽鐨勫甫鍔紝浣跨粍缁囧潡琛ㄩ潰鐨勬祦浣撲繚鎸佷氦鎹㈢姸鎬併€傚煿鍏绘恫姣忛殧鏁板ぉ鏇存崲涓€娆★紝浠ョ‘淇濊惀鍏荤墿璐ㄧ殑娴撳害骞剁Щ闄ょ粏鑳炰唬璋㈠簾鐗┿€?
鎼呮媽寮忕敓鐗╁弽搴斿櫒鐨勭己鐐规槸鎼呮媽浜х敓鐨勫壀搴斿姏浼氬缁嗚優閫犳垚涓嶅埄鐨勫奖鍝嶃€備负浜嗘洿鍔犵鍚堢粍缁囧伐绋嬬殑瑕佹眰锛岃澶氭敼鑹骇鍝佸簲杩愯€岀敓锛屽缇庡浗NBS(New Brunswick Scientific)鍏徃鐨勫崟灞傜寮忛€氭皵鎼呮媽鍣ㄣ€侹amen绛変汉鐨勫甫鐘惰灪鏃嬫〃锛屽垎鍒粠閫氭皵瑁呯疆鍜屾悈鎷屽櫒涓婃敼杩涳紝鍏嬫湇浜嗗壀搴斿姏杩囧ぇ銆佹槗浜х敓姘旀场绛変笉鍒╃粏鑳炵殑鍥犵礌銆傚緪灏忓绛夊湪鎼呮媽鍣ㄧ僵涓婁竴涓甫澶瑰鐨勭瓛浣擄紝浣垮煿鍏绘恫娴佸姩骞崇ǔ涓旇寰嬶紝骞舵惌閰嶅井瀛旈€忔皵渚涙哀绯荤粺锛岄伩鍏嶅ぇ姘旀场鐨勪骇鐢熴€備互涓婂洜绱犻兘鏄悈鎷屽紡鐢熺墿鍙嶅簲鍣ㄥ繀椤绘敼杩涚殑鏂瑰悜銆?
2.2婊氱瓛寮忕敓鐗╁弽搴斿櫒(rotating-wall vessels)
婊氱瓛寮忕敓鐗╁弽搴斿櫒鏄洰鍓嶇粍缁囧伐绋嬮鍩熸渶涓哄箍娉涘簲鐢ㄧ殑鐢熺墿鍙嶅簲鍣紝涓€鑸彲搴旂敤鍦ㄥ績琛€绠°€佽蒋楠ㄧ瓑缁嗚優鍩瑰吇銆?990骞碖leis绛変汉棣栧厛璁捐浣跨敤璇ュ瀷鐢熺墿鍙嶅簲鍣紝闅忓悗缇庡浗鍥藉澶┖鎬荤讲(NASA)搴旂敤鍒扮粍缁囧伐绋嬮鍩熴€傚叾涓昏鐗圭偣鏄煿鍏绘恫鍜岀粍缁囧潡鍦ㄥ弽搴斿櫒鍔ㄥ姏甯﹀姩涓嬶紝缁曟按骞宠酱鎴栧瀭鐩磋酱鏃嬭浆锛岄€氳繃姘旀恫涓ょ浉鐣岄潰杩涜姘斾綋浜ゆ崲锛屽苟浠ユ棤鑿屾敞灏勫櫒鎹㈡恫鏉ヨˉ鍏呭吇鍒嗐€傝浆鍔ㄤ腑鐨勫弽搴斿櫒瀵圭粍缁囧潡浜х敓浜嗘嫋鏇冲姏(Fd)銆佺蹇冨姏(Fc)锛屼互鍙婇噸鍔?Fg)绛夛紝璋冭妭鍙嶅簲鍣ㄧ殑杞€燂紝浣挎棆杞骇鐢熺殑绂诲績鍔涘垰濂藉拰缁嗚優涓庡井杞戒綋鎴栨敮鏋剁殑閲嶅姏浜掔浉骞宠 锛屽嵆鍙娇缁勭粐鍧楅殢鍙嶅簲鍣ㄦ棆杞偓娴簬鍩瑰吇娑蹭腑锛岃€屼笉鑷存帀钀界澹侊紝鏁呰鍙嶅簲鍣ㄦ彁渚涗簡鍔ㄦ€佸煿鍏荤殑鐜銆佷綆鍓簲鍔涘拰楂樼殑璐ㄤ紶閫熺巼銆侴ao绛夊婊氱瓛寮忕敓鐗╁弽搴斿櫒绯荤粺涓殑寰浇浣撹繍鍔ㄨ繘琛屾暟鍊煎垎鏋愶紝褰撳井杞戒綋棰楃矑鐨勫瘑搴﹀ぇ浜庡煿鍏诲熀瀵嗗害鏃讹紝棰楃矑浼氬悜绛掑杩佺Щ锛屽苟鎾炲嚮澹侀潰銆傛粴绛掑紡鐢熺墿鍙嶅簲鍣ㄥ緢灏戣兘鍦ㄧ嚎渚涙恫锛屼笖闅句互宸ヤ笟瑙勬ā鍖栵紝鍏跺彂灞曞彈鍒伴檺鍒躲€傚鍔犲叆鐏屾祦锛屾敼杩涘湪绾夸緵娑诧紝瀵瑰叾鍐呴儴鐜鍜屾垚鍒嗚繘琛岀簿纭帶鍒讹紝鏄鐢熺墿鍙嶅簲鍣ㄧ殑鏀硅壇鏂瑰悜銆?
2.3涓┖绾ょ淮鐢熺墿鍙嶅簲鍣?
涓┖绾ょ淮鐢熺墿鍙嶅簲鍣?hollow-fiber bioreactors)涓轰竴鐗瑰埗鐨勫渾绛掞紝閲岄潰灏佽鐫€鏁板崈鏍逛腑绌虹氦缁达紝灞炰簬涓€绉嶇粏寰殑绠＄姸缁撴瀯锛岀被浼间簬鍔ㄧ墿鐨勬瘺缁嗚绠★紝绠″鏄瀬钖勭殑鍗婇€忚啘锛屼綔涓虹粏鑳炵敓闀胯创闄勫強鍏诲垎浼犻€掔殑鐣岄潰銆傚綋鍩瑰吇娑茬敱绠℃潫涓祦杩囷紝娑蹭綋涓瘮瀛旀礊灏忕殑鍏讳唤浼氱┛鍑轰腑绌虹氦缁寸鏉熻杽鑶滐紝渚涘簲绠″璐撮檮鐨勭粏鑳炪€傜粏鑳炰唬璋骇鐗╁垯浼氭墿鏁ｈ繘鍏ョ氦缁寸鏉熻杽鑶滃唴锛岄殢鍩瑰吇娑叉祦鍑虹粏鑳炴墍鍦ㄧ殑绾ょ淮绠℃潫銆傜郴缁熷彲浜?7鈩冨煿鍏荤鎿嶄綔锛屽叾缂虹偣鏄粏鑳炵妞嶆椂浼氳緝涓嶅潎鍖€锛屽煿鍏绘恫杩涘叆绔箣缁嗚優浼氳緝澶氾紝鍚屾椂钀ュ吇鎴愬垎涔熶細鐢卞煿鍏绘恫杩涘叆绔嚦鍑哄彛绔舰鎴愰€掑噺鐨勬搴︺€傛斁澶т骇绋嬫椂锛岄€氬父浣跨敤涓€涓搷浣滃彴鎺у埗寰堝鏀腑绌虹氦缁存潫锛岃鍙嶅簲鍣ㄩ€傚悎鍩瑰吇楂樹唬璋㈢巼鍙婂鍩瑰吇鐜杈冩晱鎰熺殑缁嗚優锛屽鑲濄€佽偩绛夌粏鑳炪€傜洰鍓嶆绫诲弽搴斿櫒宸蹭綔涓轰汉宸ヨ倽涓村簥搴旂敤銆?
Kuk绛夊埄鐢ㄤ腑绌虹氦缁寸敓鐗╁弽搴斿櫒鍩瑰吇鍝轰钩鍔ㄧ墿缁嗚優锛岀粏鑳炲瘑搴﹁揪108~109cells/ml锛屽拰鍏跺畠绫诲瀷鍙嶅簲鍣ㄧ浉姣斿鍔犺繎50鍊嶃€傚湪涓€鑸腑浣庣粏鑳炲瘑搴﹀煿鍏绘椂锛屽姘ф祿搴﹁姹備笉楂橈紝鍦ㄥ弽搴斿櫒涓ぇ閮ㄥ垎浠呰棄鐢辫啘鑷敱鎵╂暎杈惧埌璐ㄤ紶鐩殑銆備絾鍦ㄩ珮瀵嗗害鍩瑰吇鏃讹紝渚涙哀鍗充笉涓€瀹氳冻澶熴€侹arel銆丳iret鍜孋ooney绛変汉鐮旂┒鏄剧ず锛屽埄鐢ㄤ腑绌虹氦缁寸敓鐗╁弽搴斿櫒鍩瑰吇楂樺瘑搴﹀摵涔冲姩鐗╃粏鑳炴椂锛屽湪绂荤氦缁寸琛ㄩ潰100~200mm澶勬湁缁嗚優鍧忔鍖恒€傚洜姝わ紝鍔犱笂鑴夊姩娴佸舰鎴愬娴佷紶閫掓槸涓€绉嶉噸瑕佺殑鏂规硶銆侲fthymiou鍜孲huler 鐮斿彂鐨勫弽搴斿櫒锛屾槸鍒╃敤鍛ㄦ湡鎬х殑鏂瑰紡锛屼娇缁嗚優浜ゆ浛涓庡煿鍏诲熀鍜岀┖姘旀帴瑙︼紝澶у箙闄嶄綆姘旀恫涓ょ浉钀ュ吇鐨勮川浼犻檺鍒讹紝姝や负鏈弽搴斿櫒鐩墠鐨勫彂灞曟柟鍚戙€?
2.4鐏屾祦鐢熺墿鍙嶅簲鍣?
鐏屾祦鐢熺墿鍙嶅簲鍣?perfusion bioreactors)鍘熺悊涓鸿繛缁坊鍔犲煿鍏绘恫鑷冲煿鍏绘Ы锛屽悓鏃朵篃鍚稿嚭鐩稿悓浣撶Н鐨勫師鏈夊煿鍏绘恫锛屼娇妲藉唴鍩瑰吇娑蹭綋绉淮鎸佷竴瀹氾紝鍏诲垎涔熻兘缁存寔鍦ㄧ粏鑳炵敓闀挎墍闇€鑼冨洿浠ヤ笂锛岀粏鑳炰唬璋骇鐗╁垯涓嶄細鍫嗙Н澶銆傚煿鍏绘椂灏嗗凡瀹屾垚绉嶆缁嗚優鐨勬敮鏋舵偓鍚婁簬鐏屾祦绯荤粺涓紝鍩瑰吇鍩虹洿鎺ユ祦杩囨敮鏋朵箣瀛旈殭锛屾祦浣撳彲鍦ㄦ敮鏋剁殑鎴潰鍧囧寑鍒嗗竷锛岃鎺ョ鐨勭粏鑳為殢涔嬪潎鍖€娉ㄥ叆澶氬瓟鏀灦涓创闄勫煿鍏汇€傜亴娴佸彲浠ユ槸寮€鏀惧紡锛岀敱绯荤粺涓畬鍏ㄦ竻闄ゆ洿鎹㈠煿鍏绘恫锛涗篃鍙互鏄瘑闂郴缁燂紝缁忕敱鍙︿竴涓煿鍏绘恫瀹瑰櫒鍐嶅惊鐜洖鍒板師鍏堜箣鍩瑰吇妲姐€傛澶栵紝鐏屾祦鍙嶅簲鍣ㄥ厠鏈嶄簡鏈烘娣峰悎浜х敓鐨勫壀搴斿姏锛屼笖鍥犱负鍩瑰吇娑插惛鍑烘椂涓嶅惈鏈夌粏鑳烇紝缁嗚優鐨勫瘑搴︿細閫愭澧炲姞鍒扮浉褰撻珮鐨勫瘑搴︺€傝嫢瀵圭粏鑳炵殑鍛ㄥ洿鐜鍖呮嫭pH鍊笺€佹俯搴︺€佸煿鍏绘恫鐨勮惀鍏绘垚鍒嗐€佷唬璋骇鐗╃瓑锛屼綔绮剧‘鐨勭洃娴嬪拰鎺у埗锛屽煿鍏荤粏鑳炵殑瀵嗗害鍙婅川閲忓彲浠ユ彁楂樸€傛绫诲弽搴斿櫒甯哥敤浜庡煿鍏婚楠肩粍缁囥€?
2.5鏂藉姞鏈烘鍔涚殑鍙嶅簲鍣?bioreactors with mechanical force)
浣撳唴涓夌淮缁勭粐缁撴瀯涓殑缁嗚優鍦ㄧ敓闀挎椂鍙楀绉嶅姏瀛﹀埡婵€锛屽叾涓簲鍔涚殑浣滅敤瀵圭粍缁囩殑缁撴瀯銆佸舰鎬佸拰鍔熻兘浜х敓鏄庢樉鐨勫奖鍝嶃€傚叧鑺傝蒋楠ㄥ湪浣撳唴姝ｅ父鐢熺悊鐘舵€佷笅鎵垮彈鐫€闂存瓏鎬х粍缁囨恫浣撳帇鍔涳紝缃楅迹J鐧藉閱ｇ殑鍚堟垚鏈夊奖鍝嶃€侭anes绛変簬1985骞磋璁′互鐪熺┖璐熷帇鎺у埗鐗靛紩鍔涚殑缁嗚優鍩瑰吇瑁呯疆銆係egurola绛夌敤榧犻楠艰倢缁嗚優鍦ㄥ簲鍔涗綔鐢ㄤ笅鍩瑰吇锛屽彂鐜颁腑绛夋按骞崇殑搴斿姏(1~12%)锛屽叾璇卞缁嗚優鎺掑垪娌垮紶搴斿姏鏂瑰悜锛涜緝澶х殑搴斿姏(12~24%)锛屽垯璇卞缁嗚優鎺掑垪寤跺瀭鐩翠簬寮犲簲鍔涙柟鍚戙€傛柦鍔犳満姊板姏鐨勫弽搴斿櫒锛屽彲璁捐鍛ㄦ湡鎬у湴瀵圭粍缁囧潡鏂藉姞搴斿姏锛屾柦鍔涚殑澶у皬鍙互鎺у埗锛屽涓鸿倢鑵辨垨闊у甫缁勭粐璁捐锛屽彲鎻愪緵寮犲姏鏉′欢鐨勫弽搴斿櫒銆?
姝ゅ锛屽皻鍙湪缁勭粐宸ョ▼鐢熺墿鍙嶅簲鍣ㄥ鍔犵數纾佸満(electromagnetic field)銆佽剦鍔ㄣ€佸啿鍑绘尝(shock wave)銆傜數纾佸満涓嶄粎鍙績杩涢鐨勫啀鐢熷拰楠ㄧ粏鑳炵殑澧炴畺锛岃繕鍙績杩涙垚楠ㄧ粏鑳炲垎娉孖GF-鈪°€丅MP-2銆乀GF-β绛夌敓闀垮洜瀛愬拰鍚堟垚缁嗚優澶栧熀璐ㄣ€備竴鑸娇鐢ㄤ箣鐢靛満寮哄害涓?0~100mA/m2锛岄鐜囧皬浜?kHz锛屼互鎻愪緵鑴夊姩鐨勫弽搴斿櫒寤烘瀯蹇冭剰鐡ｈ啘銆佷汉宸ヨ绠＄瓑銆俉ang绛夌敤鑳介噺瀵嗗害涓?.16mJ/mm2鐨勫啿鍑绘尝(鎹熶激缁嗚優鑶滆€屼笉鑷存崯浼ょ粏鑳炲櫒)浣滅敤浜庤蒋楠ㄥ熀璐ㄧ粏鑳烇紝鍙娇缁嗚優澧炴畺銆佺⒈鎬х７閰搁叾娲绘€у楂樸€佽兌鍘熻泲鐧藉強楠ㄩ挋铔嬬櫧鐨勫悎鎴愬鍔犮€傝〃涓€涓轰笉鍚岀被鍨嬬敓鐗╁弽搴斿櫒涔嬫€ц兘姣旇緝銆?
3.鐢熺墿鍙嶅簲鍣ㄧ殑缁勭粐宸ョ▼搴旂敤
缁勭粐宸ョ▼鐢熺墿鍙嶅簲鍣ㄧ殑寮€鍙戯紝浠庡彇鍑虹粍缁囧潡銆佸垎绂荤粏鑳炪€佹墿澧炲埌鏀惧ぇ鍩瑰吇杩囩▼锛岀殕閲囪嚜鍔ㄥ寲鍙婁釜浜哄寲閲忚韩璁綔鏂瑰紡銆?
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鍚勭被鍨嬬敓鐗╁弽搴斿櫒璁捐蹇呴』鑳戒豢鐢熸€у湴鎺ヨ繎浣撳唴鐜锛屽叾鍔熻兘鍜岀粨鏋勪笂灏嗘洿瓒嬪鏉傦紝骞舵湞鍚戣嚜鍔ㄥ寲銆佸鍔熻兘鍖栥€侀珮鏁堢巼鍜屽鏍峰寲鍙戝睍锛屾湡鑳藉洜搴斿悇绉嶇粍缁囷紝绮惧噯璋冩帶鐢熼暱鍥犲瓙涓庢縺绱犵殑閲婃斁锛岀ǔ瀹氭帶鍒剁敓鐗╁弽搴斿櫒鍐呴儴娓╁害銆乸H鍊煎強姘斾綋鍒嗗帇绛夊弬鏁帮紝鍙堣兘瑙嗛渶瑕佸鍩瑰吇鐨勭粍缁囨柦鍔犵墿鐞嗗弬鏁帮紝濡傜鐣咁嫳鐢电晠顙卞簲鍔涘満绛夈€傛湭鏉ワ紝鐢熺墿鍙嶅簲鍣ㄥ湪缁勭粐宸ョ▼鐨勫簲鐢ㄥ皢瀵逛汉绫绘彁渚涙洿澶氭洿澶х殑甯姪銆?
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