rGFP
Green Fluorescent Protein Recombinant
PRO-687 
Synonyms:Green fluorescent protein, GFP.   电话 13817370083  提供  50ug ，  100ug
Introduction:
rGFP, also known as Green Fluorescent Protein, is a protein produced by the jellyfish (Aequorea Victoria) that produces bioluminescence in the green zone of the noticeable spectrum. Green Fluorescent Protein is a useful and ubiquitous instrument for producing chimeric proteins, where it functions as a fluorescent protein tag. rGFP is expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. Green Fluorescent Protein permits a broad range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.

Description:
rGFP Aequorea victoria produced in E.Coli is a single, non-glycosylated polypeptide chain containing 238 amino acids (1-238 a.a.) and having a molecular mass of 26.8 kDa.
rGFP is purified by proprietary chromatographic techniques.

Source:Escherichia Coli.
Physical Appearance:Sterile filtered yellowish solution.
Formulation:The Green Fluorescent Protein solution contains 20mM Tris, pH-8 and 10% Glycerol.

Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Purity:Greater than 95.0% as determined by SDS-PAGE.

Amino Acid Sequence:
MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPEGYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD
HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK.


Green fluorescent protein, pAb        电话 13817370083  提供 100 µl
OURCE/HOST:       From rabbit.
CONCENTRATION:  1mg/ml
PURITY DETAIL:   Epitope-affinity purified.
FORMULATION:    Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:        Synthetic peptide corresponding to aa 26-39 (H26KFSVSGEGE GDAT39) of the jellyfish Aequorea victoria GFP (green fluorescent protein). This sequence is completely conserved between GFP and the modified and enhanced Blue, Green and Yellow FP variants.

SPECIFICITY:    Recognizes GFP and GFP fusion proteins. Detects a band of ~28 kDa by Western blot.

APPLICATION:   Immunocytochemistry (1:200)    ；Western Blot (1:2’000)
保 存 温 度:         -20°C
HANDLING:        Avoid freeze/thaw cycles.
描述：
Green Fluorescent Protein (GFP) is responsible for bioluminescence in the jellyfish Aequorea victoria. This protein has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through aa substitutions at specific residues.
Green Fluorescent Protein, pAb
别名：     GFP    电话 13817370083  提供   100ug
PRODUCT LINE       DNA & Protein Labelling
PRODUCT FAMILIES       AutoFluorescent Proteins
SOURCE/HOST:   From rabbit.
PURITY DETAIL:  Affinity purified.
IMMUNOGEN:   Native GFP purified from Aquorea victoria.
SPECIFICITY: Species independent.
APPLICATION: Western Blot
描述：
The green fluorescent protein (GFP) is derived from the jellyfish Aequorea victoria, that fluoresces green when exposed to blue light. GFP is a frequently used reporter for gene expression in recombinant DNA and cell transfection systems.



Blocking Peptide for PAb to Green Fluorescent Protein 

SEQUENCE:     H²⁶KFSVSGEGEGDATC³⁹.
FORMULA:       C₆₂H₉₄N₁₈O₂₅S
MW:       1523.6
PURITY: ≥95%
FORMULATION:       Lyophilized.
RECONSTITUTION: Reconstitute with 0.1ml of distilled water.
描述：
Synthetic peptide corresponding to aa 26-39 of jellyfish Aequorea victoria green fluorescent protein, with an added C-terminal cystein.
Use peptide for neutralization and control experiments with the polyclonal antibody that reacts with this product and green fluorescent protein . Using a solution with equal weights per unit volume of peptide and corresponding antibody will yield a solution with a large molar excess of peptide that is able to competitively bind the antibody.