| 植酸/总磷量检测试剂盒(Phytic Acid / Total Phosphorus Assay Kit) 50次 爱尔兰 型号:JKY/K-PHYT | 货号: |
| 名称：植酸/总磷量检测试剂盒 产品货号：K-PHYT 规格：50 产地：爱尔兰 数量：大量 外观四个玻璃小瓶和两个60毫升聚丙烯瓶 比重 不适用 在水中的溶解度易溶 pH值 7.0 气味 无 粉末和液体形式 稳定性 稳定 十年或以上(冷冻机) 成分: 名称 比例 醋酸钠缓冲液60毫升 一小瓶（聚丙烯） 甘氨酸缓冲液 一瓶（聚丙烯） 植酸酶悬浮液 一个七毫升小瓶（玻璃） 碱性磷酸酶悬浮液 一个七毫升小瓶（玻璃） 磷标准溶液25毫升 一小瓶（玻璃） 燕麦粉的控制液 一二毫升小瓶（玻璃） 试剂盒： 试剂盒适合执行50个化验可从 包含完整的检测方法，另加： 瓶1：醋酸钠缓冲液（25毫升，200毫米，pH值5.5）和 xxxx（0.02％瓦特/视频作为防腐剂）。 稳定在4 ° C的“2年 瓶2：植酸酶暂停（1.2毫升，12000单位/毫升）。 稳定在4 ° C的“2年 瓶3：甘氨酸缓冲液（25毫升，400毫米，pH值10.4），加氯化镁 （4毫米），硫酸锌（0.4毫米）和xxxx （0.02％瓦特/ 5）作为防腐剂。 稳定在4 ° C的“2年 瓶4：暂停碱性磷酸酶（1.2毫升，80单位/毫升）。 稳定在4 ° C的“2年 瓶5：磷标准溶液（24毫升，50微克/毫升）。 和xxxx（0.02％瓦特/视频作为防腐剂）。 稳定在4 ° C的“2年 瓶6：燕麦粉控制粉（5克，磷含量， 见瓶）。 稳定“在室温下5年。 Phytic Acid / Total Phosphorus A simple method for the measurement of phytic acid / total phosphorus in food and feed samples. This method does not require purification of phytic acid via anion-exchange chromatography making it amenable to high numbers of samples. Catalogue Number: K-PHYT Content: 50 assays Appearance Four glass vials and two 60 mL polypropylene containers. Specific Gravity Not applicable Solubility in Water Readily soluble. pH Value 7.0 Odour none Form powders and liquids Stability stable in a freezer for ten or more years Ingredients Name CAS Proportion Sodium acetate buffer One 60 ml vial (polypropylene) Glycine Buffer One container (polypropylene) Phytase suspension One 7 ml vial (glass) Alkaline phosphatase suspension One 7 ml vial (glass) Phosphorus standard solution One 25 ml vial (glass) Oat flour control One 12 ml vial (glass) KITS: Kits suitable for performing 50 assays are available.The kits contain the full assay method plus: Bottle 1: Sodium acetate buffer (25 mL, 200 mM, pH 5.5) and sodium azide (0.02 % w/v) as a preservative. Stable for > 2 years at 4°C. Bottle 2: Phytase suspension (1.2 mL, 12,000 U/mL). Stable for > 2 years at 4°C. Bottle 3: Glycine buffer (25 mL, 400 mM, pH 10.4), plus MgCl2 (4 mM), ZnSO4 (0.4 mM) and sodium azide (0.02 % w/v) as a preservative. Stable for > 2 years at 4°C. Bottle 4: Alkaline phosphatase suspension (1.2 mL, 80 U/mL). Stable for > 2 years at 4°C. Bottle 5: Phosphorus standard solution (24 mL, 50 μg/mL). and sodium azide (0.02 % w/v) as a preservative. Stable for > 2 years at 4°C. Bottle 6: Oat flour control powder (5 g; Phosphorus content, see bottle). Stable for > 5 years at room temperature. INTRODUCTION: Phytic acid (phytate; myo-inositol 1,2,3,4,5,6, hexakisphosphate) is the primary source of inositol and storage phosphorus in plant seeds contributing ~70 % of total phosphorus. The abundance of phytic acid in cereal grains is a concern in the foods and animal feeds industries because the phosphorus in this form is unavailable to monogastric animals due to a lack of endogenous phytases; enzymes specific for the dephosphorylation of phytic acid. In addition, the strong chelating characteristic of phytic acid reduces the bioavailability of other essential dietary nutrients such as minerals (e.g. Ca2+, Zn2+, Mg2+, Mn2+, Fe2+/3+), proteins and amino acids.1 High phytic acid content feeds are generally supplemented with inorganic phosphate, however this causes increased faecal phosphate levels and subsequent eutrophication of waterways. Alternatively, supplementation with commercial phytases is becoming increasingly popular and reduces the requirement for inorganic phosphate supplementation as well as the associated environmental issues. Currently, there is no commercially available, simple, quantitative method for phytic acid and while such measurement is relatively complex the generally accepted AOAC Method 986.11 has limitations.2 For each individual analysis the method requires cumbersome anion-exchange purification and a major inherent assumption here is that only phytic acid is purified. While this assumption is viable for non-processed grains for which phytic acid comprises at least 97 % of total inositol phosphates, it is not viable for processed foods and feeds which can contain higher levels of some lower myo-inositol phosphate forms (i.e. IP3, IP4, IP5) that would coelute with phytic acid and contribute to overestimation of the phytic acid content.3,4 Given the complexities of the purification and measurement of phytic acid separate from lower myo-inositol phosphate forms, Megazyme has developed a simple, quantitative method (K-PHYT) to measure total “available phosphorus” released from food and feed samples that is amenable to high numbers of samples and does not require tedious anion-exchange purification. This method involves acid extraction of inositol phosphates followed by treatment with a phytase that is specific for phytic acid (IP6) and the lower myo-inositol phosphate forms (i.e. IP2, IP3, IP4, IP5). Subsequent treatment with alkaline phosphatase ensures the release of the final phosphate from myoinositol phosphate (IP1) which is relatively resistant to the action of phytase. The total phosphate released is measured using a modified colorimetric method and given as grams of phosphorus per 100 g of sample material.5,6 1 Alkaline phosphatase will also release phosphate from monophosphate esters other than myo-inositol phosphate, however analyses of a broad spectrum of samples using K-PHYT indicated that phosphorus released from monophosphate esters other than myoinositol phosphate is small. 用途:矿山,食品 分类:囯际直购 历史资料:版本 版本 版本 版本 版本 版本 版本 版本 版本 |
植酸/总磷量检测试剂盒(Phytic Acid / Total Phosphorus Assay Kit) 50次 爱尔兰JKY/K-PHYT |
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