UreG鏄竴涓渶瑕佸湪鑴查叾鍚晬鐨勬椿璺冧綅鐐圭粍瑁呯殑GTP閰躲€傚湪姝わ紝涓轰簡鐮旂┒閲嶇粍澶ц偁鏉嗚弻缁嗚優涓劜閰舵縺娲讳綋鍦ㄦ椿鐨勬湁鏈轰綋鍐呯殑鍏嶇柅鏁堟灉锛岀函鍖栬泲鐧借川鐨勬€ц兘鐗瑰緛锛屾椿浣撳垎鏋愬拰浣撳铔嬬櫧璐ㄧ殑鐩镐簰浣滅敤锛屼竴涓爣鏈夐摼鐞冭弻鐨勫厠闆风櫧姘忔潌鑿?/span>UreG锛?/span>UreG_Str锛夊拰UreG_Str閫夊畾鐨勫畾鐐瑰彉绉嶈鏋勫缓銆?/span>

       閴翠簬甯︽湁鏍囩鐨勯摼鐞冭弻锛屽苟娌℃湁澧炲己UreG婵€娲昏劜閰剁殑鑳藉姏锛屽熀鏈笂搴熼櫎浜?/span>K20A锛?/span>D49A锛?/span>C72A锛?/span>H74A锛?/span>D80A锛屼互鍙?/span>S111A UreG_Str鍙樼涓叾鐨勬椿鎬э紝杩樺噺灏戜簡E25A锛?/span>C28A鍜?/span>S115A铔嬬櫧璐ㄧ殑娲绘€с€?/span> Lys20鍜?/span>Asp49鍙兘鍦?/span>GTP鐨勭粨鍚?/span>/姘磋В鍜岄晛鐨勭粨鍚堜腑鍒嗗埆鍙戞尌浣滅敤銆?/span> UreG_Str鐨勬瘡涓崟浣擄紙姣忎釜閲戝睘绂诲瓙鐨?/span>Kd涓?/span>5μM锛夊湪涓€涓粨鍚堜綅鐐圭粨鍚堜竴涓晬鎴栭攲绂诲瓙锛屽寘鎷?/span>Cys72鍦ㄥ唴锛岃繖绉嶇幇璞″拰浣跨敤C72A UreG_Str浣块晬绂诲瓙鐨?/span>Kd澧炲姞 12鍊嶅強鍦ㄧ獊鍙樿泲鐧借川涓嶅瓨鍦ㄧ殑鎯呭喌涓嬬～瀵归晬鐢佃嵎杞Щ甯︾殑鍑虹幇涓€鏍风濂囥€?/span>

       鍩轰簬UreG鍜?/span>HypB鐨勫悓婧愭€э紝涓€涓?/span>GTP閰堕渶瑕佹阿閰剁粍瑁咃紝鍚屾椂闅忕潃鍙樹綋鐨勫嚭鐜帮紝His74寰堝彲鑳芥槸涓€涓澶栫殑閲戝睘閰嶄綋銆傚湪浣撳唴鐨勬瘉鍧忓疄楠屾樉绀哄嚭Asp80瀵圭ǔ瀹?/span>UreG_Str鍜?/span>UreABC – UreDF澶嶅悎鐗╃殑鐩镐簰浣滅敤璧风潃閲嶈鐨勪綔鐢ㄣ€傚湪浣撳涓嬬殑姣佸潖瀹為獙琛ㄦ槑UreG鑳藉鍜?/span>UreE鐩哥粨鍚堬紝骞朵笖鍦ㄩ晬鎴栭攲绂诲瓙鐨勫瓨鍦ㄤ笅鐩镐簰浣滅敤澧炲己銆傞噾灞炰即渚?/span> 鏆楃ず鍦?/span>UreABC – UreDFG澶嶅悎鐗╀腑瑕佹妸鏉熺細鐨勯晬杞Щ鍒?/span>UreG涓紝鍚屾椂閲戝睘绂诲瓙闅忎箣杞Щ鍒?/span>UreD涓?/span>锛岀劧鍚庡湪GTP渚濊禆鐨勮繃绋嬩腑杞Щ鍒拌劜閰舵柊鐢熺殑娲绘€т綅鐐逛腑銆?/span>